Detailed information of TA system TA164994

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76655..76909
• Replicon: plasmid pRHB32-C22_2
• Accession: NZ_CP057221
• Organism: Escherichia fergusonii strain RHB32-C22

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HVZ07_RS22425
• Protein ID: WP_001351576.1
• Coordinates: 76655..76861 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	76848..76909 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ07_RS22395	73022..73303	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HVZ07_RS22400	73300..73569	-	270	WP_000079941.1	hypothetical protein	-
HVZ07_RS22405	74483..75340	-	858	WP_097418981.1	incFII family plasmid replication initiator RepA	-
HVZ07_RS22410	75333..75815	-	483	WP_001523473.1	hypothetical protein	-
HVZ07_RS22415	75808..75882	-	75	WP_001442103.1	RepA leader peptide Tap	-
HVZ07_RS22420	76114..76371	-	258	WP_000083845.1	replication regulatory protein RepA	-
HVZ07_RS22425	76655..76861	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	76848..76909	+	62	NuclAT_1	-	Antitoxin
-	76848..76909	+	62	NuclAT_1	-	Antitoxin
-	76848..76909	+	62	NuclAT_1	-	Antitoxin
-	76848..76909	+	62	NuclAT_1	-	Antitoxin
HVZ07_RS22430	77492..77704	-	213	WP_097418979.1	hypothetical protein	-
HVZ07_RS22435	77839..78399	-	561	WP_097418978.1	fertility inhibition protein FinO	-
HVZ07_RS22440	78454..79200	-	747	WP_097418977.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..147240	147240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T164994 WP_001351576.1 NZ_CP057221:c76861-76655 [Escherichia fergusonii]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T164994 NZ_CP073627:349413-349760 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT164994 NZ_CP057221:76848-76909 [Escherichia fergusonii]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin