Detailed information of TA system TA164979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1200555..1201234
• Replicon: chromosome
• Accession: NZ_CP057220
• Organism: Escherichia fergusonii strain RHB32-C22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: HVZ07_RS05815
• Protein ID: WP_002431667.1
• Coordinates: 1200555..1200857 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVZ07_RS05820
• Protein ID: WP_000806440.1
• Coordinates: 1200893..1201234 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ07_RS05790	1195931..1197583	+	1653	WP_181668258.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HVZ07_RS05795	1197621..1198124	-	504	WP_181668259.1	hypothetical protein	-
HVZ07_RS05800	1198121..1198921	-	801	WP_181668260.1	hypothetical protein	-
HVZ07_RS05805	1198945..1199424	-	480	WP_181668261.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HVZ07_RS05810	1199628..1200422	-	795	WP_000365145.1	TraB/GumN family protein	-
HVZ07_RS05815	1200555..1200857	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVZ07_RS05820	1200893..1201234	+	342	WP_000806440.1	HigA family addiction module antidote protein	Antitoxin
HVZ07_RS05825	1201348..1203852	-	2505	WP_181668262.1	copper-exporting P-type ATPase CopA	-
HVZ07_RS05830	1204159..1205448	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T164979 WP_002431667.1 NZ_CP057220:1200555-1200857 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T164979 NZ_CP073624:2409674-2409961 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT164979 WP_000806440.1 NZ_CP057220:1200893-1201234 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT164979 NZ_CP073624:2409435-2409674 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8