Detailed information of TA system TA164964

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 113732..114155
• Replicon: plasmid pRHB33-C04_2
• Accession: NZ_CP057216
• Organism: Escherichia fergusonii strain RHB33-C04

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A8D9PPD8
• Locus tag: HVZ08_RS23505
• Protein ID: WP_000775236.1
• Coordinates: 113994..114155 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	113732..113948 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ08_RS23475	109421..109627	+	207	WP_077782986.1	single-stranded DNA-binding protein	-
HVZ08_RS23480	109653..110195	+	543	WP_042974074.1	single-stranded DNA-binding protein	-
HVZ08_RS23485	110258..110491	+	234	WP_000005990.1	DUF905 family protein	-
HVZ08_RS23490	110557..112515	+	1959	WP_181667505.1	ParB/RepB/Spo0J family partition protein	-
HVZ08_RS23495	112570..113004	+	435	WP_032235533.1	conjugation system SOS inhibitor PsiB	-
HVZ08_RS23500	113001..113720	+	720	WP_042974070.1	plasmid SOS inhibition protein A	-
-	113732..113948	+	217	NuclAT_0	-	Antitoxin
-	113732..113948	+	217	NuclAT_0	-	Antitoxin
-	113732..113948	+	217	NuclAT_0	-	Antitoxin
-	113732..113948	+	217	NuclAT_0	-	Antitoxin
HVZ08_RS23505	113994..114155	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	Toxin
HVZ08_RS23510	114824..115030	+	207	WP_181667506.1	single-stranded DNA-binding protein	-
HVZ08_RS23515	115055..115342	+	288	WP_021569031.1	hypothetical protein	-
HVZ08_RS23520	115461..116282	+	822	WP_042973148.1	DUF945 domain-containing protein	-
HVZ08_RS23525	116579..117181	-	603	WP_072156291.1	transglycosylase SLT domain-containing protein	-
HVZ08_RS23530	117505..117888	+	384	WP_040100321.1	relaxosome protein TraM	-
HVZ08_RS23535	118083..118754	+	672	WP_040100314.1	conjugal transfer transcriptional regulator TraJ	-
HVZ08_RS23540	118892..119119	+	228	WP_072039828.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefA / pefC / pefD	1..160081	160081

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T164964 WP_000775236.1 NZ_CP057216:113994-114155 [Escherichia fergusonii]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEIRLKDGYREVTASMAYESGGK

Nucleotide

• Length: 162 bp

>T164964 NZ_CP073621:c4875076-4874900 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 217 bp

>AT164964 NZ_CP057216:113732-113948 [Escherichia fergusonii]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGCGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin