Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 85748..86609 Replicon plasmid pRHB33-C04_2
Accession NZ_CP057216
Organism Escherichia fergusonii strain RHB33-C04

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7W3G1F8
Locus tag HVZ08_RS23335 Protein ID WP_024195926.1
Coordinates 86115..86609 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7W3G1H0
Locus tag HVZ08_RS23330 Protein ID WP_001559862.1
Coordinates 85748..86092 (+) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ08_RS23295 81185..81601 - 417 WP_097418959.1 type II toxin-antitoxin system VapC family toxin -
HVZ08_RS23300 81598..81828 - 231 WP_097309516.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HVZ08_RS23305 82064..82564 + 501 WP_000528932.1 hypothetical protein -
HVZ08_RS23310 82577..83350 + 774 WP_000905949.1 hypothetical protein -
HVZ08_RS23315 83517..84650 + 1134 WP_181576151.1 DUF3800 domain-containing protein -
HVZ08_RS23320 84684..85196 - 513 WP_000151784.1 hypothetical protein -
HVZ08_RS23325 85427..85504 + 78 Protein_79 helix-turn-helix domain-containing protein -
HVZ08_RS23330 85748..86092 + 345 WP_001559862.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
HVZ08_RS23335 86115..86609 + 495 WP_024195926.1 type II toxin-antitoxin system YhaV family toxin Toxin
HVZ08_RS23340 86791..89547 + 2757 Protein_82 hypothetical protein -
HVZ08_RS23345 89599..90296 + 698 Protein_83 IS1 family transposase -
HVZ08_RS23350 90311..91375 + 1065 Protein_84 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pefA / pefC / pefD 1..160081 160081
- flank IS/Tn - - 89793..90296 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(11-147)

Antitoxin

PrlF antitoxin

(16-114)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 19275.36 Da        Isoelectric Point: 8.7864

>T164962 WP_024195926.1 NZ_CP057216:86115-86609 [Escherichia fergusonii]
MEYLEINGWKICFHECFLRQITGLMKKVAELKASKPLEYHKKKETKLLVAIERVIEERITANPLDPQFRQGNTLGEEYKH
WFRAKFLMQFRLFYRCSQEHKTIVIGWVNGFDTLRAYDSRTDAYKVFGEMLKAGNPPDDWDTLLCEAGKATATTNMVDFL
TKRI

Download         Length: 495 bp

>T164962 NZ_CP073621:4456785-4456888 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 12755.59 Da        Isoelectric Point: 4.6652

>AT164962 WP_001559862.1 NZ_CP057216:85748-86092 [Escherichia fergusonii]
MATQILNSAEVSLRAESRLTERSQTTIPAVIRDALHLKPGEYIHYTLLAGGKVIMSRQNEEQDDPVISQFLTFLENDMKK
NPQKIAPVPTMFWESMKALTAGINVDLDAPLTED

Download         Length: 345 bp

>AT164962 NZ_CP073621:c4456926-4456655 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3G1F8

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3G1H0

References