Detailed information of TA system TA164873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4978567..4979327
• Replicon: chromosome
• Accession: NZ_CP057147
• Organism: Citrobacter sp. RHB35-C21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: HVZ39_RS24055
• Protein ID: WP_019077936.1
• Coordinates: 4978567..4978878 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVZ39_RS24060
• Protein ID: WP_181633049.1
• Coordinates: 4978875..4979327 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ39_RS24025	4974234..4975136	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HVZ39_RS24030	4975133..4975768	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HVZ39_RS24035	4975765..4976694	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HVZ39_RS24040	4976731..4977105	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	-
HVZ39_RS24045	4977105..4977347	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	-
HVZ39_RS24050	4977553..4978482	+	930	WP_181633048.1	alpha/beta hydrolase	-
HVZ39_RS24055	4978567..4978878	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
HVZ39_RS24060	4978875..4979327	+	453	WP_181633049.1	helix-turn-helix domain-containing protein	Antitoxin
HVZ39_RS24065	4979345..4980286	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HVZ39_RS24070	4980331..4980768	-	438	WP_181633050.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HVZ39_RS24075	4980765..4981637	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HVZ39_RS24080	4981631..4982230	-	600	WP_016155174.1	glucose-1-phosphatase	-
HVZ39_RS24085	4982335..4983138	-	804	WP_016155173.1	DeoR/GlpR family DNA-binding transcription regulator	-
HVZ39_RS24090	4983172..4984068	-	897	WP_181633051.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T164873 WP_019077936.1 NZ_CP057147:4978567-4978878 [Citrobacter sp. RHB35-C21]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T164873 NZ_CP073361:98560-99885 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 17008.43 Da
• Isoelectric Point: 5.9259

>AT164873 WP_181633049.1 NZ_CP057147:4978875-4979327 [Citrobacter sp. RHB35-C21]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKPNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT164873 NZ_CP073361:98262-98555 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7L6U900