Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 43312..43961 Replicon chromosome
Accession NZ_CP057147
Organism Citrobacter sp. RHB35-C21

Toxin (Protein)


Gene name higB Uniprot ID A0A7L6TVT2
Locus tag HVZ39_RS00200 Protein ID WP_016157800.1
Coordinates 43312..43653 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7L6TWH1
Locus tag HVZ39_RS00205 Protein ID WP_016157799.1
Coordinates 43662..43961 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ39_RS00185 39748..41076 + 1329 WP_094168875.1 MFS transporter -
HVZ39_RS00190 41219..42610 + 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
HVZ39_RS00195 42759..43211 + 453 WP_181629548.1 DUF1198 domain-containing protein -
HVZ39_RS00200 43312..43653 + 342 WP_016157800.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HVZ39_RS00205 43662..43961 + 300 WP_016157799.1 helix-turn-helix transcriptional regulator Antitoxin
HVZ39_RS00210 44081..45265 + 1185 WP_016155040.1 purine ribonucleoside efflux pump NepI -
HVZ39_RS00215 45321..46704 - 1384 Protein_42 glycoside hydrolase family 1 protein -
HVZ39_RS00220 46797..47090 - 294 WP_019078023.1 hypothetical protein -
HVZ39_RS00225 47221..47637 + 417 WP_181633089.1 GNAT family N-acetyltransferase -
HVZ39_RS00230 47809..48627 + 819 WP_016157795.1 lipoprotein NlpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-88)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13061.92 Da        Isoelectric Point: 5.6550

>T164855 WP_016157800.1 NZ_CP057147:43312-43653 [Citrobacter sp. RHB35-C21]
MWEVETTDVFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGNPIRAFFVFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLNK

Download         Length: 342 bp

>T164855 NZ_CP073360:290453-290980 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10988.84 Da        Isoelectric Point: 8.6007

>AT164855 WP_016157799.1 NZ_CP057147:43662-43961 [Citrobacter sp. RHB35-C21]
MATLKELMAKQSPESQERIAEKVEVLRQVIALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFHL

Download         Length: 300 bp

>AT164855 NZ_CP073360:290180-290446 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6TVT2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6TWH1

References