Detailed information of TA system TA164835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 341875..342541
• Replicon: chromosome
• Accession: NZ_CP057136
• Organism: Citrobacter freundii strain RHB36-C06

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HVZ46_RS01620
• Protein ID: WP_117342464.1
• Coordinates: 342224..342541 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: HVZ46_RS01615
• Protein ID: WP_003837894.1
• Coordinates: 341875..342171 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ46_RS01600	339049..339522	-	474	WP_181618416.1	transcription elongation factor GreB	-
HVZ46_RS01605	339749..340468	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HVZ46_RS01610	340465..341817	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
HVZ46_RS01615	341875..342171	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
HVZ46_RS01620	342224..342541	-	318	WP_117342464.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVZ46_RS01625	342665..344287	-	1623	WP_181618417.1	phosphoenolpyruvate carboxykinase (ATP)	-
HVZ46_RS01630	344666..346384	+	1719	WP_181618418.1	DUF4153 domain-containing protein	-
HVZ46_RS01635	346435..347313	-	879	WP_181618419.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12226.13 Da
• Isoelectric Point: 9.8310

>T164835 WP_117342464.1 NZ_CP057136:c342541-342224 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T164835 NZ_CP073351:4196248-4196466 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT164835 WP_003837894.1 NZ_CP057136:c342171-341875 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT164835 NZ_CP073351:4195847-4196221 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5