Detailed information of TA system TA164790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1122322..1123001
• Replicon: chromosome
• Accession: NZ_CP057104
• Organism: Escherichia fergusonii strain RHB38-C01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: HVZ66_RS05560
• Protein ID: WP_002431667.1
• Coordinates: 1122322..1122624 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVZ66_RS05565
• Protein ID: WP_000806440.1
• Coordinates: 1122660..1123001 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ66_RS05535	1117698..1119350	+	1653	WP_181591242.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HVZ66_RS05540	1119388..1119891	-	504	WP_180491896.1	hypothetical protein	-
HVZ66_RS05545	1119888..1120688	-	801	WP_181591243.1	hypothetical protein	-
HVZ66_RS05550	1120712..1121191	-	480	WP_000186629.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HVZ66_RS05555	1121395..1122189	-	795	WP_181591244.1	TraB/GumN family protein	-
HVZ66_RS05560	1122322..1122624	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVZ66_RS05565	1122660..1123001	+	342	WP_000806440.1	HigA family addiction module antidote protein	Antitoxin
HVZ66_RS05570	1123115..1125619	-	2505	WP_181591245.1	copper-exporting P-type ATPase CopA	-
HVZ66_RS05575	1125926..1127215	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T164790 WP_002431667.1 NZ_CP057104:1122322-1122624 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T164790 NZ_CP073286:c58140-57829 [Klebsiella pneumoniae subsp. ozaenae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT164790 WP_000806440.1 NZ_CP057104:1122660-1123001 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT164790 NZ_CP073286:c57832-57413 [Klebsiella pneumoniae subsp. ozaenae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8