Detailed information of TA system TA164721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1287828..1288507
• Replicon: chromosome
• Accession: NZ_CP057093
• Organism: Escherichia fergusonii strain RHB38-C07

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0A1A1R1
• Locus tag: HVZ69_RS06320
• Protein ID: WP_000057541.1
• Coordinates: 1287828..1288130 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVZ69_RS06325
• Protein ID: WP_077900377.1
• Coordinates: 1288166..1288507 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ69_RS06295	1283204..1284856	+	1653	WP_181588487.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HVZ69_RS06300	1284894..1285397	-	504	WP_000667001.1	hypothetical protein	-
HVZ69_RS06305	1285394..1286194	-	801	WP_181588488.1	hypothetical protein	-
HVZ69_RS06310	1286218..1286697	-	480	WP_000186629.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HVZ69_RS06315	1286901..1287695	-	795	WP_181588489.1	TraB/GumN family protein	-
HVZ69_RS06320	1287828..1288130	+	303	WP_000057541.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVZ69_RS06325	1288166..1288507	+	342	WP_077900377.1	HigA family addiction module antidote protein	Antitoxin
HVZ69_RS06330	1288565..1291069	-	2505	WP_181588490.1	copper-exporting P-type ATPase CopA	-
HVZ69_RS06335	1291376..1292665	+	1290	WP_181588491.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11795.37 Da
• Isoelectric Point: 10.2638

>T164721 WP_000057541.1 NZ_CP057093:1287828-1288130 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T164721 NZ_CP073111:976962-977363 [Agrobacterium rhizogenes]
ATGCCGCGCTATATGCTCGATACGAATATGTGCATCTATCTGATGAAGAACCAGCCCGAGCAAGTCGCTCGTCGTTTTGC
CGCTTGCTACGTCGGTGACGTGGTGATGTCGGCCATTACCTTCGCGGAGCTGGATTACGGTGTTGCCGCGTCTGATGCCC
CGGAACGGGAGCGCGGCAACCTTGATGCCTTGGTGACCCTCATTGCGGTAGAGCCGTTCGATATCGCCGCTGCCCACGCT
TATGGCCCAGTACGCATGGCAACGCGAGAGCGCAAGAAAGACCATCTGGACAAGCTGATCGCATCACACGCCATTGCCTT
GAATACGGTGCTTGTTACCAACAACACACGAGATTTCATGGCCTATCCCAACCTCAAAATCGAAAACTGGCTGGATACCT
GA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13205.12 Da
• Isoelectric Point: 5.7790

>AT164721 WP_077900377.1 NZ_CP057093:1288166-1288507 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDFKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT164721 NZ_CP073111:976726-976962 [Agrobacterium rhizogenes]
ATGCAGATGACCAAGGCATTCAAGAACGGCAATTCCCAAGCCGTACGCATCCCAGCAGAGATTGCCTATGAGCGCACGGA
CATGGAGCTGGAGATTGAGCGGGTAGGCGATGAAATCCGCATTCGTCCTGTCCGCCAGTCGTTGGCCGGCGTCATGGATG
TTTTCGCACGCTTTTCCCCCAATTTCATGAGCGAGGGCAGGGGTGACCAAGAGCAGGACGAGCGCGAGAAGATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A1A1R1