Detailed information of TA system TA164717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 612652..613451
• Replicon: chromosome
• Accession: NZ_CP057093
• Organism: Escherichia fergusonii strain RHB38-C07

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: HVZ69_RS02970
• Protein ID: WP_000347270.1
• Coordinates: 612652..613116 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HVZ69_RS02975
• Protein ID: WP_181588313.1
• Coordinates: 613116..613451 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ69_RS02940	607653..608087	-	435	WP_181588311.1	PTS sugar transporter subunit IIA	-
HVZ69_RS02945	608105..608983	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HVZ69_RS02950	608973..609752	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HVZ69_RS02955	609763..610236	-	474	WP_032303829.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HVZ69_RS02960	610259..611539	-	1281	WP_181588312.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HVZ69_RS02965	611788..612597	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HVZ69_RS02970	612652..613116	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HVZ69_RS02975	613116..613451	-	336	WP_181588313.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HVZ69_RS02980	613600..615171	-	1572	WP_181588314.1	galactarate dehydratase	-
HVZ69_RS02985	615642..616412	+	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
HVZ69_RS02990	616437..617327	+	891	WP_000178100.1	2-hydroxy-3-oxopropionate reductase	-
HVZ69_RS03000	617982..618350	-	369	WP_181588315.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T164717 WP_000347270.1 NZ_CP057093:c613116-612652 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T164717 NZ_CP073110:3032939-3033220 [Agrobacterium rhizogenes]
ATGACCTATGAGCTGGGCTTTCTGCATGCCGCTTTAAAAGAGTGGCGAAAGCTCGATGCCAATACGCGGGAGCAATTTAA
GAAGAAGCTTGCCGAGCGCTGCGAAAACCCGCGAGTACCGTCCGCCAAACTTTTCGGAAGCAAAGACCGGTACAAGATCA
AATTGCGCGGCGTCGGCTATCGGCTTGTCTATGAAGTTCGCGACGAGCAGCTCGTGATTATCGTCGTGGCTGTCGGGAAG
AGAGAGCGAAACGAAGTTTATAGAGCTGCCGATAGGCGCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12282.88 Da
• Isoelectric Point: 4.3398

>AT164717 WP_181588313.1 NZ_CP057093:c613451-613116 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEEEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT164717 NZ_CP073110:3032701-3032949 [Agrobacterium rhizogenes]
ATGACAAATGTAATTCTCGCTGAAGTGACCGCCAGCGTATCGGAATTGAAAAAGAACCCAATGGGAACCGTGGCAGCCGG
CGAAGGGGCGACGGTTGCTATTCTCAACAGGAATGCGCCTGCATTCTATTGTGTCCCGGCCCGCCTCTATGAAGCGATGC
TGGATCGTCTTGAGGATATGGAACTCAACGCCATCGCCGACGCGCGTGCGGGCGAGCCCATCATCAAGGTCAAGCTGGAT
GACCTATGA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90