Detailed information of TA system TA164669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 40990..41717
• Replicon: plasmid pRHB39-C24_2
• Accession: NZ_CP057035
• Organism: Klebsiella pneumoniae strain RHB39-C24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HVZ90_RS25475
• Protein ID: WP_011251285.1
• Coordinates: 40990..41301 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVZ90_RS25480
• Protein ID: WP_011251286.1
• Coordinates: 41298..41717 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ90_RS25445	36746..38182	+	1437	WP_149358977.1	PLP-dependent aminotransferase family protein	-
HVZ90_RS25450	38344..38703	-	360	Protein_39	LysR family transcriptional regulator	-
HVZ90_RS25455	38910..39503	-	594	Protein_40	IS66 family transposase zinc-finger binding domain-containing protein	-
HVZ90_RS25460	39552..39899	-	348	WP_149358976.1	IS66 family insertion sequence element accessory protein TnpB	-
HVZ90_RS25465	39896..40234	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
HVZ90_RS25470	40327..40785	+	459	Protein_43	DDE-type integrase/transposase/recombinase	-
HVZ90_RS25475	40990..41301	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HVZ90_RS25480	41298..41717	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HVZ90_RS25485	41864..42832	+	969	WP_181578647.1	IS5 family transposase	-
HVZ90_RS25490	42887..43258	-	372	Protein_47	transposase	-
HVZ90_RS25495	43290..43625	-	336	Protein_48	conjugal transfer protein TraI	-
HVZ90_RS25500	43639..44427	-	789	WP_023288262.1	hypothetical protein	-
HVZ90_RS25505	44448..44891	-	444	WP_181578650.1	hypothetical protein	-
HVZ90_RS25510	45488..46263	+	776	Protein_51	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..227199	227199
	-	inside	IScluster/Tn	-	-	26309..49418	23109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T164669 WP_011251285.1 NZ_CP057035:40990-41301 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T164669 NZ_CP073060:477007-477396 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATAAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTACTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT164669 WP_011251286.1 NZ_CP057035:41298-41717 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT164669 NZ_CP073060:476743-477000 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1