Detailed information of TA system TA164624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1274711..1275390
• Replicon: chromosome
• Accession: NZ_CP056968
• Organism: Escherichia fergusonii strain RHB41-C15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0A1A1R1
• Locus tag: HV010_RS06240
• Protein ID: WP_000057541.1
• Coordinates: 1274711..1275013 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV010_RS06245
• Protein ID: WP_077900377.1
• Coordinates: 1275049..1275390 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV010_RS06215	1270087..1271739	+	1653	WP_181588487.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HV010_RS06220	1271777..1272280	-	504	WP_000667001.1	hypothetical protein	-
HV010_RS06225	1272277..1273077	-	801	WP_181588488.1	hypothetical protein	-
HV010_RS06230	1273101..1273580	-	480	WP_000186629.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HV010_RS06235	1273784..1274578	-	795	WP_181588489.1	TraB/GumN family protein	-
HV010_RS06240	1274711..1275013	+	303	WP_000057541.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV010_RS06245	1275049..1275390	+	342	WP_077900377.1	HigA family addiction module antidote protein	Antitoxin
HV010_RS06250	1275448..1277952	-	2505	WP_181588490.1	copper-exporting P-type ATPase CopA	-
HV010_RS06255	1278259..1279548	+	1290	WP_181588491.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11795.37 Da
• Isoelectric Point: 10.2638

>T164624 WP_000057541.1 NZ_CP056968:1274711-1275013 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T164624 NZ_CP073002:4174814-4175032 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13205.12 Da
• Isoelectric Point: 5.7790

>AT164624 WP_077900377.1 NZ_CP056968:1275049-1275390 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDFKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT164624 NZ_CP073002:4174413-4174787 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A1A1R1