Detailed information of TA system TA164574

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1920049..1920274
• Replicon: chromosome
• Accession: NZ_CP056961
• Organism: Escherichia fergusonii strain RHB41-C20

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: HV012_RS09395
• Protein ID: WP_000813254.1
• Coordinates: 1920119..1920274 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1920049..1920107 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV012_RS09355	1915226..1915444	-	219	WP_001171946.1	hypothetical protein	-
HV012_RS09360	1915604..1915759	-	156	WP_000379575.1	DUF1391 family protein	-
HV012_RS09365	1916052..1916390	-	339	WP_001397087.1	peptidase S24-like family protein	-
HV012_RS09370	1916782..1917024	+	243	WP_000747951.1	hypothetical protein	-
HV012_RS09375	1917008..1917433	+	426	WP_000693867.1	Rha family transcriptional regulator	-
HV012_RS09380	1917505..1918575	+	1071	WP_096488174.1	phage replisome organizer	-
HV012_RS09385	1918616..1919041	+	426	WP_001151224.1	DUF977 family protein	-
HV012_RS09390	1919216..1919881	+	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
-	1920049..1920107	-	59	-	-	Antitoxin
HV012_RS09395	1920119..1920274	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
HV012_RS09400	1920442..1920714	+	273	WP_032348564.1	hypothetical protein	-
HV012_RS09405	1920716..1921765	+	1050	WP_024190197.1	DUF968 domain-containing protein	-
HV012_RS09410	1921778..1922137	+	360	WP_000904122.1	RusA family crossover junction endodeoxyribonuclease	-
HV012_RS09415	1922134..1922823	+	690	WP_001064894.1	antiterminator	-
HV012_RS09440	1923620..1923835	+	216	WP_000839572.1	class II holin family protein	-
HV012_RS09445	1923840..1924154	+	315	WP_000193273.1	YdfR family protein	-
HV012_RS09450	1924210..1924743	+	534	WP_001274714.1	lysozyme	-
HV012_RS09455	1924960..1925145	+	186	WP_001228685.1	Rz1 family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1900698..1947645	46947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T164574 WP_000813254.1 NZ_CP056961:1920119-1920274 [Escherichia fergusonii]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T164574 NZ_CP072986:c1806670-1806296 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT164574 NZ_CP056961:c1920107-1920049 [Escherichia fergusonii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin