Detailed information of TA system TA164572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1276049..1276728
• Replicon: chromosome
• Accession: NZ_CP056961
• Organism: Escherichia fergusonii strain RHB41-C20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0A1A1R1
• Locus tag: HV012_RS06250
• Protein ID: WP_000057541.1
• Coordinates: 1276049..1276351 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV012_RS06255
• Protein ID: WP_077900377.1
• Coordinates: 1276387..1276728 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV012_RS06225	1271425..1273077	+	1653	WP_181588487.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HV012_RS06230	1273115..1273618	-	504	WP_000667001.1	hypothetical protein	-
HV012_RS06235	1273615..1274415	-	801	WP_181588488.1	hypothetical protein	-
HV012_RS06240	1274439..1274918	-	480	WP_000186629.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HV012_RS06245	1275122..1275916	-	795	WP_181588489.1	TraB/GumN family protein	-
HV012_RS06250	1276049..1276351	+	303	WP_000057541.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV012_RS06255	1276387..1276728	+	342	WP_077900377.1	HigA family addiction module antidote protein	Antitoxin
HV012_RS06260	1276786..1279290	-	2505	WP_181588490.1	copper-exporting P-type ATPase CopA	-
HV012_RS06265	1279597..1280886	+	1290	WP_181588491.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11795.37 Da
• Isoelectric Point: 10.2638

>T164572 WP_000057541.1 NZ_CP056961:1276049-1276351 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T164572 NZ_CP072986:1794526-1794777 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13205.12 Da
• Isoelectric Point: 5.7790

>AT164572 WP_077900377.1 NZ_CP056961:1276387-1276728 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDFKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT164572 NZ_CP072986:1794774-1795028 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0A1A1R1