Detailed information of TA system TA164555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2544095..2544755
• Replicon: chromosome
• Accession: NZ_CP056951
• Organism: Escherichia fergusonii strain RHB42-C01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV015_RS12225
• Protein ID: WP_046077353.1
• Coordinates: 2544402..2544755 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV015_RS12220
• Protein ID: WP_001553123.1
• Coordinates: 2544095..2544397 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV015_RS12200	2539454..2540251	+	798	WP_046077351.1	DgsA anti-repressor MtfA	-
HV015_RS12210	2540589..2541850	+	1262	Protein_2380	integrase arm-type DNA-binding domain-containing protein	-
HV015_RS12215	2542707..2543591	-	885	WP_046077352.1	integrase domain-containing protein	-
HV015_RS12220	2544095..2544397	-	303	WP_001553123.1	XRE family transcriptional regulator	Antitoxin
HV015_RS12225	2544402..2544755	-	354	WP_046077353.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV015_RS12230	2545252..2545473	-	222	WP_046077354.1	helix-turn-helix domain-containing protein	-
HV015_RS12235	2545470..2545937	-	468	WP_046077355.1	hypothetical protein	-
HV015_RS12240	2546824..2547603	+	780	WP_046077393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13486.42 Da
• Isoelectric Point: 8.9507

>T164555 WP_046077353.1 NZ_CP056951:c2544755-2544402 [Escherichia fergusonii]
VWTIKTTDMFDHWFISLNDTDRASVLAALLVLREKGPGLSRPYADTIRGSRYSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPIADREFTNWLNTLKEKE

Nucleotide

• Length: 354 bp

>T164555 NZ_CP072980:c3843844-3843742 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11084.82 Da
• Isoelectric Point: 5.1786

>AT164555 WP_001553123.1 NZ_CP056951:c2544397-2544095 [Escherichia fergusonii]
MGRTLEQLIADEKPEVVDEAQAMATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT164555 NZ_CP072980:3843704-3843972 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT