Detailed information of TA system TA164510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2544097..2544757
• Replicon: chromosome
• Accession: NZ_CP056916
• Organism: Escherichia fergusonii strain RHB42-C24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV025_RS12225
• Protein ID: WP_046077353.1
• Coordinates: 2544404..2544757 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV025_RS12220
• Protein ID: WP_001553123.1
• Coordinates: 2544097..2544399 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV025_RS12200	2539456..2540253	+	798	WP_046077351.1	DgsA anti-repressor MtfA	-
HV025_RS12210	2540591..2541852	+	1262	Protein_2380	integrase arm-type DNA-binding domain-containing protein	-
HV025_RS12215	2542709..2543593	-	885	WP_046077352.1	integrase domain-containing protein	-
HV025_RS12220	2544097..2544399	-	303	WP_001553123.1	XRE family transcriptional regulator	Antitoxin
HV025_RS12225	2544404..2544757	-	354	WP_046077353.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV025_RS12230	2545254..2545475	-	222	WP_046077354.1	helix-turn-helix domain-containing protein	-
HV025_RS12235	2545472..2545939	-	468	WP_046077355.1	hypothetical protein	-
HV025_RS12240	2546826..2547605	+	780	WP_046077393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13486.42 Da
• Isoelectric Point: 8.9507

>T164510 WP_046077353.1 NZ_CP056916:c2544757-2544404 [Escherichia fergusonii]
VWTIKTTDMFDHWFISLNDTDRASVLAALLVLREKGPGLSRPYADTIRGSRYSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPIADREFTNWLNTLKEKE

Nucleotide

• Length: 354 bp

>T164510 NZ_CP072970:834610-835020 [Enterobacter sp. BWH 37]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTGGTGTTTGATAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGGGATCTGCGCCGGCTAATCGTGCAACAGCCAA
CGCAGGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11084.82 Da
• Isoelectric Point: 5.1786

>AT164510 WP_001553123.1 NZ_CP056916:c2544399-2544097 [Escherichia fergusonii]
MGRTLEQLIADEKPEVVDEAQAMATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT164510 NZ_CP072970:834363-834629 [Enterobacter sp. BWH 37]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA