Detailed information of TA system TA164469

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42058..42322
• Replicon: plasmid pRHBSTW-00011_3
• Accession: NZ_CP056909
• Organism: Citrobacter freundii strain RHBSTW-00011

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: HV032_RS26355
• Protein ID: WP_001303307.1
• Coordinates: 42170..42322 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	42058..42120 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV032_RS26340	37297..39588	-	2292	WP_001289276.1	hypothetical protein	-
HV032_RS26345	39581..40651	-	1071	WP_021013257.1	IncI1-type conjugal transfer protein TrbB	-
HV032_RS26350	40670..41878	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	42058..42120	-	63	NuclAT_0	-	Antitoxin
-	42058..42120	-	63	NuclAT_0	-	Antitoxin
-	42058..42120	-	63	NuclAT_0	-	Antitoxin
-	42058..42120	-	63	NuclAT_0	-	Antitoxin
HV032_RS26355	42170..42322	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
HV032_RS26360	42394..42645	-	252	WP_001291965.1	hypothetical protein	-
HV032_RS26365	43689..43898	-	210	WP_001140543.1	hemolysin expression modulator Hha	-
HV032_RS26370	43996..44658	-	663	WP_021013261.1	plasmid IncI1-type surface exclusion protein ExcA	-
HV032_RS26375	44729..46897	-	2169	WP_000698368.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..95042	95042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T164469 WP_001303307.1 NZ_CP056909:42170-42322 [Citrobacter freundii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T164469 NZ_CP072949:322962-323330 [Klebsiella pneumoniae MGH 39]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTATTAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT164469 NZ_CP056909:c42120-42058 [Citrobacter freundii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin