Detailed information of TA system TA164460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2701338..2701900
• Replicon: chromosome
• Accession: NZ_CP056907
• Organism: Citrobacter freundii strain RHBSTW-00011

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV032_RS13000
• Protein ID: WP_181634717.1
• Coordinates: 2701338..2701616 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV032_RS13005
• Protein ID: WP_043016302.1
• Coordinates: 2701616..2701900 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV032_RS12990	2698035..2698937	-	903	WP_043016304.1	endonuclease/exonuclease/phosphatase family protein	-
HV032_RS12995	2699402..2701117	+	1716	WP_100194375.1	ATP-binding cassette domain-containing protein	-
HV032_RS13000	2701338..2701616	+	279	WP_181634717.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV032_RS13005	2701616..2701900	+	285	WP_043016302.1	HigA family addiction module antidote protein	Antitoxin
HV032_RS13010	2701944..2702546	-	603	WP_181633738.1	inorganic diphosphatase	-
HV032_RS13015	2702676..2703332	-	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
HV032_RS13020	2703325..2704209	-	885	WP_003833024.1	formate dehydrogenase N subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10651.26 Da
• Isoelectric Point: 9.8965

>T164460 WP_181634717.1 NZ_CP056907:2701338-2701616 [Citrobacter freundii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRPAVIDAASKVTDINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T164460 NZ_CP072945:675970-676311 [Enterobacter sp. MGH 7]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10357.07 Da
• Isoelectric Point: 8.8094

>AT164460 WP_043016302.1 NZ_CP056907:2701616-2701900 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT164460 NZ_CP072945:675632-675949 [Enterobacter sp. MGH 7]
ATGAGCAACACCACATGGGGACTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTTCAGGAGGGCAACTGGCT
GCACTATTTGGCTGACCGAGCCAGTATTACCGGTAAATTCAGCGAAGCAGAATGTCTTAAGCTGGATGTGGTATTTCCGA
ATTTTATCAGCCAGTTGGAGTCAATGCTTACCACTGGTGAAATGAACCCTAGCCATGCCTATTGTGTCACCCTGTACCAC
AATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGCCGTTTACCCCACTCAACGTTAA