Detailed information of TA system TA164445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3084948..3085608
• Replicon: chromosome
• Accession: NZ_CP056899
• Organism: Citrobacter sp. RHBSTW-00017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2I8S535
• Locus tag: HV037_RS15020
• Protein ID: WP_053390179.1
• Coordinates: 3085255..3085608 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV037_RS15015
• Protein ID: WP_103283718.1
• Coordinates: 3084948..3085250 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV037_RS15000	3081616..3082878	+	1263	WP_103283720.1	integrase arm-type DNA-binding domain-containing protein	-
HV037_RS15005	3083560..3084447	-	888	WP_103283719.1	integrase domain-containing protein	-
HV037_RS15010	3084579..3084881	+	303	WP_146053242.1	hypothetical protein	-
HV037_RS15015	3084948..3085250	-	303	WP_103283718.1	XRE family transcriptional regulator	Antitoxin
HV037_RS15020	3085255..3085608	-	354	WP_053390179.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV037_RS15025	3086107..3086328	-	222	WP_103283717.1	helix-turn-helix domain-containing protein	-
HV037_RS15030	3086325..3086792	-	468	WP_103283716.1	hypothetical protein	-
HV037_RS15035	3087312..3088292	-	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
HV037_RS15040	3088620..3089317	-	698	Protein_2953	IS1 family transposase	-
HV037_RS15045	3089372..3089689	+	318	Protein_2954	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13442.36 Da
• Isoelectric Point: 9.0134

>T164445 WP_053390179.1 NZ_CP056899:c3085608-3085255 [Citrobacter sp. RHBSTW-00017]
VWTIKTTDMFDHWFSSLNDIDRASVLAALLVLREKGPGLSRPYADTLRGSRFSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMIPVADREFTNWLNTIKEKE

Nucleotide

• Length: 354 bp

>T164445 NZ_CP072933:1069784-1070263 [Xylella fastidiosa subsp. multiplex]
ATGCAGATTTCGACACCTCATTCACTCACGGCAGCGCATCGACTTGATGAATTCAATTGCGGTGAGCCATCGCTGGATGA
TTGGCTTAAACGACGCGCACTGACCAACCATTTAAACGGGGCCAGCCGGACCTTCGTGGTTATCGATGCCAACCAATACG
TGCTTGGGTACTACGCTTTGGCAGCCGGGGCTGTAGCGCATCAAGAGGCCACCCGTGCTATCCGACGTAACATGCCTGAT
CCAGTGCCGGTCATGGTGCTCGGAAGGCTGGCTGTGGATACTCGTGCTCAAGGCATCAAACTCGGTGCGGCCTTGCTTAA
AGATACGGTAACTCGTGTGCAGTCCATTGCAGAGAACGCCGGCGTCAGGGCATTGGTAGTCCATGCGTTGAACGAGCGCG
CAAAGCAATTCTACGAGTACTATGGTTTTCGAGCGTCACCAATGCACCTGATGACCTTGATGCTTCCGATTAAATTCTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11054.88 Da
• Isoelectric Point: 6.4738

>AT164445 WP_103283718.1 NZ_CP056899:c3085250-3084948 [Citrobacter sp. RHBSTW-00017]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAKALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDIELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT164445 NZ_CP072933:1069509-1069781 [Xylella fastidiosa subsp. multiplex]
ATGCGCGACGCTTCCATCAACTTACGTGCCATGCCTGAGCAGCGCGACTTGATTGACCAGGCTGCTAATCTATTAGGCAA
GAATCGCTCAGACTTCATGCTAGAAGCTGCGTGCGAGCGTGCGAAAGCAATTATCCTGGATCAAGTCTTTTTCAATCTGG
ACGAAGAGAGGTTCCGGCAATTCACTGTGTTGCTTAATGCGCCACAAGACACCAATCCAGGCCTTAATCGCCTGATGGCA
ATTAAGGCTCCGTGGAGCATCAGCAATACGTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S535