Detailed information of TA system TA164424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3047370..3048030
• Replicon: chromosome
• Accession: NZ_CP056896
• Organism: Citrobacter sp. RHBSTW-00021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2I8S535
• Locus tag: HV039_RS14845
• Protein ID: WP_053390179.1
• Coordinates: 3047677..3048030 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV039_RS14840
• Protein ID: WP_103283718.1
• Coordinates: 3047370..3047672 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV039_RS14825	3044038..3045300	+	1263	WP_103283720.1	integrase arm-type DNA-binding domain-containing protein	-
HV039_RS14830	3045982..3046869	-	888	WP_103283719.1	integrase domain-containing protein	-
HV039_RS14835	3047001..3047303	+	303	WP_146053242.1	hypothetical protein	-
HV039_RS14840	3047370..3047672	-	303	WP_103283718.1	XRE family transcriptional regulator	Antitoxin
HV039_RS14845	3047677..3048030	-	354	WP_053390179.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV039_RS14850	3048529..3048750	-	222	WP_103283717.1	helix-turn-helix domain-containing protein	-
HV039_RS14855	3048747..3049214	-	468	WP_103283716.1	hypothetical protein	-
HV039_RS14860	3049734..3050714	-	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
HV039_RS14865	3051042..3051739	-	698	Protein_2918	IS1 family transposase	-
HV039_RS14870	3051794..3052111	+	318	Protein_2919	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3047370..3056187	8817

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13442.36 Da
• Isoelectric Point: 9.0134

>T164424 WP_053390179.1 NZ_CP056896:c3048030-3047677 [Citrobacter sp. RHBSTW-00021]
VWTIKTTDMFDHWFSSLNDIDRASVLAALLVLREKGPGLSRPYADTLRGSRFSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMIPVADREFTNWLNTIKEKE

Nucleotide

• Length: 354 bp

>T164424 NZ_CP072914:84529-85104 [Klebsiella oxytoca]
ATGCGACATTCACCTTATCCGGTGATTTTAGATGCCTGTGTGTTATATCCGGCACGACTCAGAGATTTGCTGATGCACCT
GGGGATTGCCGGGCTGTATCAACCAAAATGGAGCCGCTGCATTCAGGATGAATGGTGCAGAAACTTGATTGCTAATCGGC
CTGACATTCTGCCAGAGACGCTTCAGCGTACTGTCAAAATGATGAATTCCGCCTTACCAGATGCCAATGTTACGGGATAT
AAGGGATTAATAAGTAATCTGCATCTGCCGGATCCCAACGACAGACATGTTCTTGCCGCAGCGATAAGAGCGAAAGCTGA
AATTATTGTAACGCTTAACCATAAGGACTTTCCGCCAGAACTTCTGGGTCCATATGAAATAGAAACGCTTCACCCTGATG
CGTTTATTTCCGATCTCTTCGATCTTAATCATGCTCTGACGCTTGAAGCCGTCAGGCGGCAGCGACTGTCTCTAAAACAT
CCATCTATGGCGGTCGATGAATTTCTTGAAATGCTGCTAAAGCAGGGTTTACCGATGACGGTAAAGGAATTAGCGCATTA
TCAGTATGCTATTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11054.88 Da
• Isoelectric Point: 6.4738

>AT164424 WP_103283718.1 NZ_CP056896:c3047672-3047370 [Citrobacter sp. RHBSTW-00021]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAKALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDIELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT164424 NZ_CP072914:84074-84529 [Klebsiella oxytoca]
ATGACTACACTGACTCATCTCAACCTTCCCGATCGCCAGGAGAGCGAACTTGCGCAGCGCGGACAGCGTGAGCTTGCGGC
GTATCTTTCCACCAGGCTGGAAACACAGCGTATTGCCATTGTAGGAGAGGATGATAAAGCTCATACCATAGAGCTACCTA
CAAGTGCGCTGACATTGCTGATGGAGATATTGGGGGAGTTGGCTAGCGGAAATGCGGTACAAATCGTTCCCGTACATGCG
GAACTCACAACCCAGGAGGCGGCAAATATACTCAATGTCTCTCGTCCGCATATGGTTAAGCTCCTGGAAGCGAGGAAGCT
CCCATTTCATAAAACGGGGCGTCATCGCAGAGTGTTGTTTGCCGATCTTATGGAGTATAAGAAACGCAGGGAACAGGAGA
GCCTGGACGCGATGCAATCATTGGCCGATCAGGCGCAGGACTTAGGGATGTATTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S535