Detailed information of TA system TA164319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2680892..2681454
• Replicon: chromosome
• Accession: NZ_CP056850
• Organism: Citrobacter freundii strain RHBSTW-00120

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV079_RS13060
• Protein ID: WP_096757033.1
• Coordinates: 2680892..2681170 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV079_RS13065
• Protein ID: WP_096757032.1
• Coordinates: 2681170..2681454 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV079_RS13040	2676001..2676432	-	432	WP_181636782.1	OsmC family protein	-
HV079_RS13045	2676661..2676801	+	141	WP_096757036.1	stationary-phase-induced ribosome-associated protein	-
HV079_RS13050	2676981..2678678	+	1698	WP_181636783.1	malate dehydrogenase	-
HV079_RS13055	2678989..2680704	+	1716	WP_181636784.1	ATP-binding cassette domain-containing protein	-
HV079_RS13060	2680892..2681170	+	279	WP_096757033.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV079_RS13065	2681170..2681454	+	285	WP_096757032.1	HigA family addiction module antidote protein	Antitoxin
HV079_RS13070	2681559..2682215	-	657	WP_096757031.1	formate dehydrogenase-N subunit gamma	-
HV079_RS13075	2682208..2683092	-	885	WP_115258486.1	formate dehydrogenase N subunit beta	-
HV079_RS13080	2683106..2686153	-	3048	WP_117342799.1	formate dehydrogenase-N subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10601.05 Da
• Isoelectric Point: 9.5320

>T164319 WP_096757033.1 NZ_CP056850:2680892-2681170 [Citrobacter freundii]
MIMNFRHKGLRDLFLQGRTSGVTAKHVRRLRHRLAVIDAASHVTDINMPGYKLHALTGDRDGVWSISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T164319 NZ_CP072896:c24210-23347 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10327.05 Da
• Isoelectric Point: 8.8094

>AT164319 WP_096757032.1 NZ_CP056850:2681170-2681454 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAQ
KSVDISRLRKLVAQ

Nucleotide

• Length: 285 bp

>AT164319 NZ_CP072896:c24484-24212 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA