Detailed information of TA system TA164292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 322401..323067
• Replicon: chromosome
• Accession: NZ_CP056839
• Organism: Citrobacter freundii strain RHBSTW-00126

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B5Q945
• Locus tag: HV083_RS01490
• Protein ID: WP_003847996.1
• Coordinates: 322750..323067 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV083_RS01485
• Protein ID: WP_048218780.1
• Coordinates: 322401..322697 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV083_RS01470	319577..320050	-	474	WP_003023524.1	transcription elongation factor GreB	-
HV083_RS01475	320276..320995	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HV083_RS01480	320992..322344	+	1353	WP_003837891.1	two-component system sensor histidine kinase EnvZ	-
HV083_RS01485	322401..322697	-	297	WP_048218780.1	helix-turn-helix domain-containing protein	Antitoxin
HV083_RS01490	322750..323067	-	318	WP_003847996.1	hypothetical protein	Toxin
HV083_RS01495	323190..324812	-	1623	WP_003023529.1	phosphoenolpyruvate carboxykinase (ATP)	-
HV083_RS01500	325191..326909	+	1719	WP_048218779.1	DUF4153 domain-containing protein	-
HV083_RS01505	327014..327892	-	879	WP_003023531.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12225.15 Da
• Isoelectric Point: 10.0909

>T164292 WP_003847996.1 NZ_CP056839:c323067-322750 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Nucleotide

• Length: 318 bp

>T164292 NZ_CP072869:c47711-47292 [Rhizobium sp. NLR16a]
ATGGCGCGTTACATGCTCGATACGAACATGTGTATCTACCTGATGAAAAACCAGCCGGAGCAGGTCGCTCGCCGCTTTGC
CAGTTGCTATGTGGGGGACGTGGTGATGTCCGCCATCACCTTCGCGGAACTCGAGTACGGTGTTTCCGCATCCGATGATC
CGGCACGAGAACTTGATAATCTCACCGCCCTGGCCGAGCTGATCACGGTAGAGCCGTTTGGGATCGCAGCAGCGCGTGCC
TACGGTCCGGTTCGAATGGCGACGAGAGACCGGAAGAAAGATCATCTCGACAAACTGATCGCATCGCATGCGATCGCTCT
GGACACGGTATTGGTGACCAACAACACGCGGGACTTCCTGGCGTATCCCGGACTGAAGCTCGAAAACTGGCTGGACGCGG
AACGCAGAGAAACCAATTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11185.99 Da
• Isoelectric Point: 7.2050

>AT164292 WP_048218780.1 NZ_CP056839:c322697-322401 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKVI

Nucleotide

• Length: 297 bp

>AT164292 NZ_CP072869:c47947-47711 [Rhizobium sp. NLR16a]
ATGCACACCACTAAAGCTTTCAAGAATGGAAATTCCCAGGCGGTTCGCATACCGGCGGAACTGGCGTTTGAGCGAACCGA
TGTTGAGTTGGAAATCGAGCGCATCGGCGACGAGCTTCGCATCCGTCCTGTGCATCGCTCCCTGGCCGGCGTTCTGGATG
TATTCGCCCGCTTCTCTCCGGATTTCATGCGCGACGGAAGAGAGTCCCATGAGCAAGAGGAGCGGGATAAGATCTGA

Structures

Toxin

• AlphaFold DB: A0A8B5Q945