Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4913068..4913828 Replicon chromosome
Accession NZ_CP056834
Organism Citrobacter sp. RHBSTW-00127

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3E2Q1
Locus tag HV084_RS23655 Protein ID WP_047357780.1
Coordinates 4913068..4913379 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7W3E2R1
Locus tag HV084_RS23660 Protein ID WP_047357781.1
Coordinates 4913376..4913828 (+) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV084_RS23625 4908735..4909637 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV084_RS23630 4909634..4910269 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV084_RS23635 4910266..4911195 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV084_RS23640 4911232..4911606 - 375 WP_016155182.1 type II toxin-antitoxin system VapC family toxin -
HV084_RS23645 4911606..4911848 - 243 WP_016155181.1 ribbon-helix-helix domain-containing protein -
HV084_RS23650 4912054..4912983 + 930 WP_094169176.1 alpha/beta hydrolase -
HV084_RS23655 4913068..4913379 + 312 WP_047357780.1 type II toxin-antitoxin system HigB family toxin Toxin
HV084_RS23660 4913376..4913828 + 453 WP_047357781.1 helix-turn-helix domain-containing protein Antitoxin
HV084_RS23665 4913846..4914787 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV084_RS23670 4914832..4915269 - 438 WP_016157874.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV084_RS23675 4915266..4916138 - 873 WP_019077938.1 virulence factor BrkB family protein -
HV084_RS23680 4916132..4916731 - 600 WP_016157873.1 glucose-1-phosphatase -
HV084_RS23685 4916999..4918027 - 1029 WP_047357782.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(95-148)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12545.70 Da        Isoelectric Point: 10.1490

>T164289 WP_047357780.1 NZ_CP056834:4913068-4913379 [Citrobacter sp. RHBSTW-00127]
MHVISRKPFNEARLRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Download         Length: 312 bp

>T164289 NZ_CP072865:c3059792-3059523 [Rhizobium sp. NLR16a]
ATGGCTTGGAGAATTGAGTTTGACCGGGCAGCCGAGCGTGAGCTTGAGAAGCTTGGACATGAGGCGGCTCGCCGAATCCT
CCGGTTTCTCAATGATCGCGTTGCCAAGCTGGACGATCCACGATCCATCGGAGAGGCGCTTAAAGGATCTGAACTTGGGG
ACTTCTGGAAGTACCGCATCGGGGACTATCGGGTGATCGCAAGCATCGACGATGGAACCGTGCGAATCCTCATCGTGCGG
ATTGGCAATCGCCGTGATGTCTATCGATGA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16986.38 Da        Isoelectric Point: 6.1421

>AT164289 WP_047357781.1 NZ_CP056834:4913376-4913828 [Citrobacter sp. RHBSTW-00127]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQHGLKAADLANELGSKSNVSNILNGRRALTISHIKMLAERFNLPADAFIE

Download         Length: 453 bp

>AT164289 NZ_CP072865:c3060000-3059776 [Rhizobium sp. NLR16a]
ATGTTGGCCTTGCGGTTGCCACCTGAAATCGAAGCACGCCTCGATGAGCTTGCCAGGCGCACGGGCCGCAGCAAGAGCTT
CTACGCGCGTCAAGCCATTCTCGAACATCTCGATGATCTCGAGGATGTCTATCTCGCCGAAAAGCGCCTCGAAGAGCTTC
GCCGAGGTGAGAGCGATACAGTGCCGCTGGCCGAGCTGATGGCACGTCATGGCTTGGAGAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E2Q1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3E2R1

References