Detailed information of TA system TA164252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4636014..4636684
• Replicon: chromosome
• Accession: NZ_CP056809
• Organism: Citrobacter freundii strain RHBSTW-00162

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: HV103_RS22190
• Protein ID: WP_046671374.1
• Coordinates: 4636382..4636684 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV103_RS22185
• Protein ID: WP_046671375.1
• Coordinates: 4636014..4636355 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV103_RS22155	4631555..4632313	+	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-
HV103_RS22160	4632403..4633083	+	681	WP_046671387.1	phosphonate C-P lyase system protein PhnL	-
HV103_RS22165	4633080..4634216	+	1137	WP_003031811.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HV103_RS22170	4634219..4634773	+	555	WP_003844764.1	ribose 1,5-bisphosphokinase	-
HV103_RS22175	4634760..4635194	+	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
HV103_RS22180	4635203..4635961	+	759	WP_046671376.1	phosphonate metabolism protein PhnP	-
HV103_RS22185	4636014..4636355	-	342	WP_046671375.1	HigA family addiction module antidote protein	Antitoxin
HV103_RS22190	4636382..4636684	-	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV103_RS22195	4636840..4637169	-	330	WP_003031807.1	hypothetical protein	-
HV103_RS22200	4637239..4639503	-	2265	WP_044702173.1	hybrid sensor histidine kinase/response regulator	-
HV103_RS22205	4639618..4641141	+	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T164252 WP_046671374.1 NZ_CP056809:c4636684-4636382 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T164252 NZ_CP072850:391318-391851 [Vibrio cholerae MO10]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT164252 WP_046671375.1 NZ_CP056809:c4636355-4636014 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT164252 NZ_CP072850:391052-391321 [Vibrio cholerae MO10]
ATGGCAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTT
AAAAAGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGG
TTAAAGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTT
AAATTTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6