164236

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 328821..329487 Replicon chromosome
Accession NZ_CP056809
Organism Citrobacter freundii strain RHBSTW-00162

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag HV103_RS01540 Protein ID WP_003847996.1
Coordinates 329170..329487 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag HV103_RS01535 Protein ID WP_003837894.1
Coordinates 328821..329117 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV103_RS01520 325997..326470 - 474 WP_003023524.1 transcription elongation factor GreB -
HV103_RS01525 326696..327415 + 720 WP_001157751.1 two-component system response regulator OmpR -
HV103_RS01530 327412..328764 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
HV103_RS01535 328821..329117 - 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
HV103_RS01540 329170..329487 - 318 WP_003847996.1 hypothetical protein Toxin
HV103_RS01545 329610..331232 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
HV103_RS01550 331611..333329 + 1719 WP_046671282.1 DUF4153 domain-containing protein -
HV103_RS01555 333439..334317 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T164236 WP_003847996.1 NZ_CP056809:c329487-329170 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T164236 NZ_CP072848:431454-431723 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT164236 WP_003837894.1 NZ_CP056809:c329117-328821 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT164236 NZ_CP072848:431717-432238 [Vibrio cholerae]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References