Detailed information of TA system TA164212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 7005..7864
• Replicon: plasmid p2
• Accession: NZ_CP056778
• Organism: Enterobacter cloacae strain DSM 30054

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A8B3V8K3
• Locus tag: HWQ16_RS27035
• Protein ID: WP_013087279.1
• Coordinates: 7005..7499 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A8B3VF40
• Locus tag: HWQ16_RS27040
• Protein ID: WP_013087280.1
• Coordinates: 7520..7864 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ16_RS27005	2901..3119	+	219	WP_013087271.1	hypothetical protein	-
HWQ16_RS27010	3511..4269	-	759	WP_013087274.1	hypothetical protein	-
HWQ16_RS27015	4432..5280	-	849	WP_013087275.1	DUF4942 domain-containing protein	-
HWQ16_RS27020	5470..6150	-	681	WP_013087276.1	hypothetical protein	-
HWQ16_RS27025	6247..6495	-	249	WP_044160311.1	hypothetical protein	-
HWQ16_RS27030	6809..7003	+	195	WP_139348812.1	hypothetical protein	-
HWQ16_RS27035	7005..7499	-	495	WP_013087279.1	type II toxin-antitoxin system YhaV family toxin	Toxin
HWQ16_RS27040	7520..7864	-	345	WP_013087280.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
HWQ16_RS27045	8186..8404	-	219	WP_013087281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HWQ16_RS27050	8644..8874	-	231	WP_013087283.1	hypothetical protein	-
HWQ16_RS27055	8865..9128	-	264	WP_013087284.1	DUF1380 family protein	-
HWQ16_RS27060	9235..9516	-	282	WP_044160304.1	hypothetical protein	-
HWQ16_RS27065	9664..10182	-	519	WP_013087286.1	hypothetical protein	-
HWQ16_RS27070	10172..10546	-	375	WP_044160300.1	hypothetical protein	-
HWQ16_RS27075	10558..10905	-	348	WP_079972020.1	hypothetical protein	-
HWQ16_RS27080	10934..11365	-	432	WP_013087289.1	antirestriction protein	-
HWQ16_RS27085	11731..11919	+	189	WP_013087290.1	hypothetical protein	-
HWQ16_RS27090	12182..12619	-	438	WP_013087292.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86414	86414

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (11-147)

Antitoxin

• PrlF antitoxin (16-114)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 19174.06 Da
• Isoelectric Point: 8.6417

>T164212 WP_013087279.1 NZ_CP056778:c7499-7005 [Enterobacter cloacae]
MEYLELNGWKICFHKCFLDQIAELAQKVAELKAAKPNEYHKKKETKLLIAIERVIEEWIASDPLNPQYRQGDTLGDEYKH
WFRVKFLQQFRLFYRCSQEHKTIIIGWVNDFDTLRAYGSKTDAYKVFAEMLKAGTPPDDWNTLLNAAKKATATNTIPGFL
TRQP

Nucleotide

• Length: 495 bp

>T164212 NZ_CP072842:1989981-1990238 [Faecalibacter bovis]
ATGAAATATATTTTCGTAGATGAATCTTGGGAAGATTATTTATTTTGGCAAAAAACCGATAAGAAAAAAGTAAAACGTAT
CAATGATTTATTGAAAGATATTTCACGTACTCCTTTTACTGGTATAGGGAAACCAGAGCCATTAAAACATAAATATTCAG
GGTTTTGGTCACGTAGAATAGATGAAGAACATCGTTTAATTTACAAGTACGAAGACGGTCAGATATTAATAGCAAAGTGC
CGTTTTCATTACGATTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12515.27 Da
• Isoelectric Point: 4.4335

>AT164212 WP_013087280.1 NZ_CP056778:c7864-7520 [Enterobacter cloacae]
MATQMLNSADVSLRAESRLTERSQTTIPAAIRDALHLKPGESIHYTLLAGGKVIMSKKEEEQDDPVVPQFLAFLENDMKK
NPQNIAPVPALFWESIKALTAGVEVDLDAPLTDD

Nucleotide

• Length: 345 bp

>AT164212 NZ_CP072842:1989726-1989980 [Faecalibacter bovis]
ATGATAATAGCAAGTGTTTCAGATTTCAGAAAAGATATTAAATCTTACTTAGATAGAGTTTCGAAGAATTTTGAAACCTT
AATCATCAACAGAGGGAAAGATTCAGGTATTGTGGTAATGTCATTAGAAGAGTACAATTCTTTAATGGCTACAAATTACG
AATTATCTTCTCGTATCAATGAATCAAGATTAGATGCTGCCATTGAAAAATTAAAAAGTAACCAATCTTTTGAAAGAGAA
TTAATCGAGGAATAG

Structures

Toxin

• AlphaFold DB: A0A8B3V8K3

Antitoxin

• AlphaFold DB: A0A8B3VF40