Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 400478..401127 Replicon chromosome
Accession NZ_CP056776
Organism Enterobacter cloacae strain DSM 30054

Toxin (Protein)


Gene name higB Uniprot ID A0A5E1A383
Locus tag HWQ16_RS01980 Protein ID WP_013097692.1
Coordinates 400478..400840 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5E1A3P3
Locus tag HWQ16_RS01985 Protein ID WP_013097693.1
Coordinates 400828..401127 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ16_RS01960 395812..396783 - 972 WP_013097688.1 LacI family transcriptional regulator -
HWQ16_RS01965 396859..398001 - 1143 WP_013097689.1 Gfo/Idh/MocA family oxidoreductase -
HWQ16_RS01970 397994..399019 - 1026 WP_013097690.1 sugar phosphate isomerase/epimerase -
HWQ16_RS01975 399035..400273 - 1239 WP_013097691.1 MFS transporter -
HWQ16_RS01980 400478..400840 + 363 WP_013097692.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HWQ16_RS01985 400828..401127 + 300 WP_013097693.1 helix-turn-helix transcriptional regulator Antitoxin
HWQ16_RS01990 401253..402641 - 1389 WP_013097694.1 phenylalanine transporter -
HWQ16_RS01995 402744..404717 - 1974 WP_013097695.1 PhoX family phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 390590..401127 10537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13994.00 Da        Isoelectric Point: 7.3235

>T164190 WP_013097692.1 NZ_CP056776:400478-400840 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Download         Length: 363 bp

>T164190 NZ_CP072825:c5377475-5376942 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10875.62 Da        Isoelectric Point: 8.6152

>AT164190 WP_013097693.1 NZ_CP056776:400828-401127 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Download         Length: 300 bp

>AT164190 NZ_CP072825:c5377752-5377486 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A383

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1A3P3

References