Detailed information of TA system TA164121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 51648..52294
• Replicon: chromosome
• Accession: NZ_CP056751
• Organism: Enterobacter hormaechei strain RHBSTW-00216

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV128_RS00280
• Protein ID: WP_181522317.1
• Coordinates: 51648..51998 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7H8UAV4
• Locus tag: HV128_RS00285
• Protein ID: WP_045335986.1
• Coordinates: 51995..52294 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV128_RS00245	47325..47795	+	471	WP_015703840.1	DNA repair protein RadC	-
HV128_RS00250	47808..48134	+	327	WP_059443453.1	type IV toxin-antitoxin system YeeU family antitoxin	-
HV128_RS00255	48155..48475	+	321	WP_059443702.1	TA system toxin CbtA family protein	-
HV128_RS00260	48770..50326	+	1557	WP_047050722.1	hypothetical protein	-
HV128_RS00265	50366..50542	-	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
HV128_RS00270	50679..51020	+	342	WP_006812446.1	SymE family type I addiction module toxin	-
HV128_RS00275	51123..51375	+	253	Protein_54	DNA-binding protein	-
HV128_RS00280	51648..51998	+	351	WP_181522317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV128_RS00285	51995..52294	+	300	WP_045335986.1	XRE family transcriptional regulator	Antitoxin
HV128_RS00290	52359..53240	-	882	WP_047050719.1	helix-turn-helix domain-containing protein	-
HV128_RS00295	53483..53962	-	480	WP_047050718.1	hypothetical protein	-
HV128_RS00300	53964..54752	-	789	WP_045329485.1	winged helix-turn-helix domain-containing protein	-
HV128_RS00305	55587..56189	+	603	WP_047050717.1	response regulator transcription factor	-
HV128_RS00310	56242..56826	+	585	WP_023299002.1	type 1 fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	45104..66662	21558

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13482.62 Da
• Isoelectric Point: 8.8416

>T164121 WP_181522317.1 NZ_CP056751:51648-51998 [Enterobacter hormaechei]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSLYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTLHLTSLEAAK

Nucleotide

• Length: 351 bp

>T164121 NZ_CP072793:2018617-2018943 [Thiothrix unzii]
ATGAGTACAACATTCAGCCGTTACGATACTGCTGCCTATTTAAAGACCGATGAAGATATGCAGGACTATCTGGAAGCTTG
CATGGAAGAGGCGGGAGATGATCCGCAATTCATTGCCAAAGCGTTGGGCGTGATTGCCCGCGCCCGTGGAATGTCACAAC
TGGCACGTGATTCCGGGTTGGCACGTGAGAGTCTTTACAAGGCGTTATCGGGTGAAGGTAATCCTGAGTTTTCCACTATT
CTGAAAGTGATTAACGCTATGGGGTTACGATTGCATTTCAGCTTGCGGGACACCGCACCCACTCTCTCAACGGTGAAACT
TTCGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11129.87 Da
• Isoelectric Point: 6.4714

>AT164121 WP_045335986.1 NZ_CP056751:51995-52294 [Enterobacter hormaechei]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVADLMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT164121 NZ_CP072793:2018315-2018620 [Thiothrix unzii]
ATGATAGAAATTATCCAAAGTAAGGTGTTTTCAGATTGGCTGCTTGGTCTGAAAGATCGACAGGCGCGGGCGCGTATTTA
TGCGCGGCTTGATCGCATGGCGGATGGAAACTTTGGTGATGCCGAACCGATAGGTGAAGGAATGAGTGAGGCACGCATTC
ATTATGGCCCCGGTTATCGGTTATATTTCATGCAACGGGGGCAGGCATTGGTAGTGCTGTTGTGTGGTGGTGACAAGTCC
ACCCAACAGCGTGATATTCAACGAGCAAAGGTAGTTGCCGATCTGTGGAAGGGGGAAAATCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7H8UAV4