Detailed information of TA system TA164061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1236739..1237388
• Replicon: chromosome
• Accession: NZ_CP056737
• Organism: Enterobacter roggenkampii strain RHBSTW-00309

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV150_RS05935
• Protein ID: WP_106106301.1
• Coordinates: 1237026..1237388 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1S2AD04
• Locus tag: HV150_RS05930
• Protein ID: WP_047746098.1
• Coordinates: 1236739..1237038 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV150_RS05920	1233163..1235136	+	1974	WP_106106303.1	PhoX family phosphatase	-
HV150_RS05925	1235240..1236628	+	1389	WP_106106302.1	phenylalanine transporter	-
HV150_RS05930	1236739..1237038	-	300	WP_047746098.1	helix-turn-helix transcriptional regulator	Antitoxin
HV150_RS05935	1237026..1237388	-	363	WP_106106301.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV150_RS05940	1237595..1238830	+	1236	WP_021241592.1	MFS transporter	-
HV150_RS05945	1238846..1239871	+	1026	WP_106106300.1	sugar phosphate isomerase/epimerase	-
HV150_RS05950	1239864..1241006	+	1143	WP_106106299.1	Gfo/Idh/MocA family oxidoreductase	-
HV150_RS05955	1241082..1242053	+	972	WP_021241589.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13936.81 Da
• Isoelectric Point: 5.1960

>T164061 WP_106106301.1 NZ_CP056737:c1237388-1237026 [Enterobacter roggenkampii]
MWNVETTDRFDDWFFEQTTALKEDVLATMHILAEFGPTLGRPYVDTVKDSAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMIVLADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T164061 NZ_CP072790:c699856-699464 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10906.70 Da
• Isoelectric Point: 9.8627

>AT164061 WP_047746098.1 NZ_CP056737:c1237038-1236739 [Enterobacter roggenkampii]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTLAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT164061 NZ_CP072790:c700110-699853 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1S2AD04