Detailed information of TA system TA164027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1242046..1242773
• Replicon: chromosome
• Accession: NZ_CP056719
• Organism: Enterobacter hormaechei strain RHBSTW-00333

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9E6WR45
• Locus tag: HV163_RS06065
• Protein ID: WP_045623285.1
• Coordinates: 1242462..1242773 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV163_RS06060
• Protein ID: WP_045623284.1
• Coordinates: 1242046..1242465 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV163_RS06025	1237643..1237870	+	228	WP_124304273.1	hypothetical protein	-
HV163_RS06030	1238672..1238866	+	195	Protein_1168	hypothetical protein	-
HV163_RS06035	1238879..1239730	+	852	Protein_1169	RHS domain-containing protein	-
HV163_RS06040	1239727..1240209	+	483	WP_063139119.1	immunity 26/phosphotriesterase HocA family protein	-
HV163_RS06045	1240423..1240722	+	300	WP_124304274.1	hypothetical protein	-
HV163_RS06050	1240726..1240968	-	243	WP_124304275.1	hypothetical protein	-
HV163_RS06055	1241332..1241778	+	447	WP_045623318.1	hypothetical protein	-
HV163_RS06060	1242046..1242465	-	420	WP_045623284.1	helix-turn-helix domain-containing protein	Antitoxin
HV163_RS06065	1242462..1242773	-	312	WP_045623285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV163_RS06070	1242994..1243404	-	411	WP_023299534.1	ASCH domain-containing protein	-
HV163_RS06075	1243605..1244060	-	456	WP_023305643.1	hypothetical protein	-
HV163_RS06080	1244764..1244976	+	213	WP_022647355.1	RNA chaperone/antiterminator CspA	-
HV163_RS06085	1245203..1246099	-	897	WP_045623287.1	LysR family transcriptional regulator	-
HV163_RS06090	1246205..1246984	+	780	WP_088581799.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12453.32 Da
• Isoelectric Point: 9.8331

>T164027 WP_045623285.1 NZ_CP056719:c1242773-1242462 [Enterobacter hormaechei]
MPAVSRAPFDAATRHFPIQELALEDLYWVLKGEHYPSPDDMRKRFPSRERMKYREKWWVIDVGGGYLRVMLFTDFERGKL
FIKHTSTHADYDKLTDLYRRKKE

Nucleotide

• Length: 312 bp

>T164027 NZ_CP072786:c3997367-3996960 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15522.56 Da
• Isoelectric Point: 4.7722

>AT164027 WP_045623284.1 NZ_CP056719:c1242465-1242046 [Enterobacter hormaechei]
MNFSAAIKAANDLTRELPFLGSSPSRQDYEDAMGLVEYLIEHDPDSPLVEMLTAKIDKYENESPEFAEFNARVASIPSGV
ALLRTLMDQYHLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGVQVSAFVGN

Nucleotide

• Length: 420 bp

>AT164027 NZ_CP072786:c3997614-3997348 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA