Detailed information of TA system TA164025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4937269..4938029
• Replicon: chromosome
• Accession: NZ_CP056717
• Organism: Citrobacter sp. RHBSTW-00599

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV243_RS23855
• Protein ID: WP_053388923.1
• Coordinates: 4937269..4937577 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV243_RS23860
• Protein ID: WP_053388924.1
• Coordinates: 4937577..4938029 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV243_RS23825	4932937..4933839	+	903	WP_053388921.1	formate dehydrogenase O subunit beta	-
HV243_RS23830	4933836..4934471	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HV243_RS23835	4934468..4935397	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HV243_RS23840	4935434..4935808	-	375	WP_053388922.1	type II toxin-antitoxin system VapC family toxin	-
HV243_RS23845	4935808..4936050	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	-
HV243_RS23850	4936255..4937184	+	930	WP_149330683.1	alpha/beta hydrolase	-
HV243_RS23855	4937269..4937577	+	309	WP_053388923.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV243_RS23860	4937577..4938029	+	453	WP_053388924.1	helix-turn-helix domain-containing protein	Antitoxin
HV243_RS23865	4938047..4938988	-	942	WP_053388925.1	fatty acid biosynthesis protein FabY	-
HV243_RS23870	4939033..4939470	-	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV243_RS23875	4939467..4940339	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HV243_RS23880	4940333..4940932	-	600	WP_016157873.1	glucose-1-phosphatase	-
HV243_RS23885	4941023..4941913	-	891	WP_053388926.1	aldose epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4936255..4949617	13362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 12373.55 Da
• Isoelectric Point: 10.1496

>T164025 WP_053388923.1 NZ_CP056717:4937269-4937577 [Citrobacter sp. RHBSTW-00599]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHK

Nucleotide

• Length: 309 bp

>T164025 NZ_CP072786:c3077421-3077170 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 17014.39 Da
• Isoelectric Point: 5.9258

>AT164025 WP_053388924.1 NZ_CP056717:4937577-4938029 [Citrobacter sp. RHBSTW-00599]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNEAEYRKALALVEYLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVTLLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT164025 NZ_CP072786:c3077173-3076919 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA