Detailed information of TA system TA163956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 97279..97925
• Replicon: chromosome
• Accession: NZ_CP056707
• Organism: Enterobacter hormaechei strain RHBSTW-00564

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV236_RS00500
• Protein ID: WP_047050720.1
• Coordinates: 97575..97925 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7H8UAV4
• Locus tag: HV236_RS00495
• Protein ID: WP_045335986.1
• Coordinates: 97279..97578 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV236_RS00470	92747..93331	-	585	WP_023299002.1	type 1 fimbrial protein	-
HV236_RS00475	93384..93986	-	603	WP_047050717.1	response regulator transcription factor	-
HV236_RS00480	94821..95609	+	789	WP_045329485.1	winged helix-turn-helix domain-containing protein	-
HV236_RS00485	95611..96090	+	480	WP_047050718.1	hypothetical protein	-
HV236_RS00490	96333..97214	+	882	WP_047050719.1	helix-turn-helix domain-containing protein	-
HV236_RS00495	97279..97578	-	300	WP_045335986.1	XRE family transcriptional regulator	Antitoxin
HV236_RS00500	97575..97925	-	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV236_RS00505	98198..98450	-	253	Protein_100	DNA-binding protein	-
HV236_RS00515	99815..100258	+	444	WP_181522242.1	hypothetical protein	-
HV236_RS00520	100359..101339	-	981	WP_000019450.1	IS5-like element ISKpn26 family transposase	-
HV236_RS00525	101408..101839	+	432	Protein_104	Eco57I restriction-modification methylase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	94821..119359	24538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13497.59 Da
• Isoelectric Point: 8.8416

>T163956 WP_047050720.1 NZ_CP056707:c97925-97575 [Enterobacter hormaechei]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTLHLTSLEAAK

Nucleotide

• Length: 351 bp

>T163956 NZ_CP072769:259783-260133 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11129.87 Da
• Isoelectric Point: 6.4714

>AT163956 WP_045335986.1 NZ_CP056707:c97578-97279 [Enterobacter hormaechei]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVADLMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT163956 NZ_CP072769:259491-259778 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7H8UAV4