Detailed information of TA system TA163930

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85392..85815
• Replicon: plasmid pRHBSTW-00605_2
• Accession: NZ_CP056698
• Organism: Escherichia marmotae strain RHBSTW-00605

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: HV246_RS23775
• Protein ID: WP_000775238.1
• Coordinates: 85392..85553 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85599..85815 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV246_RS23735	80406..80726	+	321	WP_181479303.1	transglycosylase SLT domain-containing protein	-
HV246_RS23740	80800..81972	+	1173	WP_181479304.1	IS21 family transposase	-
HV246_RS23745	81972..82769	+	798	WP_000544830.1	IS21-like element IS21 family helper ATPase IstB	-
HV246_RS23750	82831..82992	+	162	Protein_86	transglycosylase SLT domain-containing protein	-
HV246_RS23755	83271..84092	-	822	WP_063097562.1	DUF945 domain-containing protein	-
HV246_RS23760	84199..84495	-	297	WP_001470485.1	hypothetical protein	-
HV246_RS23765	84519..84725	-	207	WP_069894508.1	single-stranded DNA-binding protein	-
HV246_RS23770	84795..85091	+	297	Protein_90	hypothetical protein	-
HV246_RS23775	85392..85553	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	85599..85815	-	217	NuclAT_0	-	Antitoxin
-	85599..85815	-	217	NuclAT_0	-	Antitoxin
-	85599..85815	-	217	NuclAT_0	-	Antitoxin
-	85599..85815	-	217	NuclAT_0	-	Antitoxin
HV246_RS23780	85827..86546	-	720	WP_176261377.1	plasmid SOS inhibition protein A	-
HV246_RS23785	86543..86977	-	435	WP_001558309.1	conjugation system SOS inhibitor PsiB	-
HV246_RS23790	87046..89004	-	1959	WP_181479305.1	ParB/RepB/Spo0J family partition protein	-
HV246_RS23795	89068..89301	-	234	WP_000006026.1	DUF905 domain-containing protein	-
HV246_RS23800	89358..89813	-	456	WP_181479306.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..127911	127911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T163930 WP_000775238.1 NZ_CP056698:c85553-85392 [Escherichia marmotae]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T163930 NZ_CP072765:c2237580-2237236 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Nucleotide

• Length: 217 bp

>AT163930 NZ_CP056698:c85815-85599 [Escherichia marmotae]
TCACACGGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin