Detailed information of TA system TA163889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 53806..54530
• Replicon: plasmid pRHBSTW-00634_6
• Accession: NZ_CP056687
• Organism: Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: HV252_RS31655
• Protein ID: WP_023292165.1
• Coordinates: 54219..54530 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV252_RS31650
• Protein ID: WP_020801939.1
• Coordinates: 53806..54222 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV252_RS31620	49152..49460	-	309	WP_071599372.1	hypothetical protein	-
HV252_RS31625	49520..50341	-	822	WP_020804822.1	DUF945 domain-containing protein	-
HV252_RS31630	51167..52000	-	834	WP_020804845.1	type I restriction-modification system subunit M	-
HV252_RS31635	52049..52195	-	147	WP_004152750.1	hypothetical protein	-
HV252_RS31640	52290..52637	-	348	WP_020804846.1	hypothetical protein	-
HV252_RS31645	52704..53084	-	381	WP_020802391.1	hypothetical protein	-
HV252_RS31650	53806..54222	-	417	WP_020801939.1	helix-turn-helix domain-containing protein	Antitoxin
HV252_RS31655	54219..54530	-	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV252_RS31660	54637..54861	-	225	WP_042922612.1	hypothetical protein	-
HV252_RS31665	54872..55078	-	207	WP_042922554.1	hypothetical protein	-
HV252_RS31670	55715..56065	-	351	WP_004152758.1	hypothetical protein	-
HV252_RS31675	56062..56334	-	273	WP_042922555.1	hypothetical protein	-
HV252_RS31680	56692..57414	-	723	WP_020804573.1	plasmid SOS inhibition protein A	-
HV252_RS31685	57411..57842	-	432	WP_020804574.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76134	76134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T163889 WP_023292165.1 NZ_CP056687:c54530-54219 [Klebsiella grimontii]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T163889 NZ_CP072764:3188378-3188821 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15380.49 Da
• Isoelectric Point: 4.5542

>AT163889 WP_020801939.1 NZ_CP056687:c54222-53806 [Klebsiella grimontii]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVG

Nucleotide

• Length: 417 bp

>AT163889 NZ_CP072764:3188134-3188391 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3