Detailed information of TA system TA163870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1947434..1948094
• Replicon: chromosome
• Accession: NZ_CP056682
• Organism: Klebsiella grimontii strain RHBSTW-00634

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A285B6D8
• Locus tag: HV252_RS09230
• Protein ID: WP_004132737.1
• Coordinates: 1947434..1947787 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A9E1DQ94
• Locus tag: HV252_RS09235
• Protein ID: WP_004132739.1
• Coordinates: 1947792..1948094 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV252_RS09205	1942818..1943585	-	768	WP_004132727.1	molybdopterin-dependent oxidoreductase	-
HV252_RS09210	1943575..1944183	-	609	WP_004132729.1	cytochrome b/b6 domain-containing protein	-
HV252_RS09215	1944198..1944824	-	627	WP_004132731.1	hypothetical protein	-
HV252_RS09220	1944964..1945632	+	669	WP_004132733.1	heavy metal response regulator transcription factor	-
HV252_RS09225	1945632..1947050	+	1419	WP_181511743.1	heavy metal sensor histidine kinase	-
HV252_RS09230	1947434..1947787	+	354	WP_004132737.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV252_RS09235	1947792..1948094	+	303	WP_004132739.1	XRE family transcriptional regulator	Antitoxin
HV252_RS09240	1948185..1948355	+	171	WP_165909793.1	helix-turn-helix domain-containing protein	-
HV252_RS09245	1948478..1948681	+	204	Protein_1811	hypothetical protein	-
HV252_RS09250	1948682..1949015	+	334	Protein_1812	type II toxin-antitoxin system PemK/MazF family toxin	-
HV252_RS09255	1949192..1949872	-	681	WP_181511744.1	hypothetical protein	-
HV252_RS09260	1949975..1950661	-	687	WP_181511745.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13554.49 Da
• Isoelectric Point: 10.0045

>T163870 WP_004132737.1 NZ_CP056682:1947434-1947787 [Klebsiella grimontii]
VWMIKTTDTFERWFTSFNDTDRASVLAALLVLREKGPGLSRPNADTLRGSRYSNMKELRIQSRGEPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPVAEREFTNWLKTFKEKE

Nucleotide

• Length: 354 bp

>T163870 NZ_CP072764:c2236229-2235669 [Mycobacterium tuberculosis]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11072.83 Da
• Isoelectric Point: 5.7424

>AT163870 WP_004132739.1 NZ_CP056682:1947792-1948094 [Klebsiella grimontii]
MGRTLEQLMADEKPEVVADARAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFEL

Nucleotide

• Length: 303 bp

>AT163870 NZ_CP072764:c2236567-2236226 [Mycobacterium tuberculosis]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Structures

Toxin

• AlphaFold DB: A0A285B6D8