Detailed information of TA system TA163842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1191490..1192217
• Replicon: chromosome
• Accession: NZ_CP056663
• Organism: Enterobacter hormaechei strain RHBSTW-00668

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9E6WR45
• Locus tag: HV259_RS05620
• Protein ID: WP_045623285.1
• Coordinates: 1191906..1192217 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV259_RS05615
• Protein ID: WP_045623284.1
• Coordinates: 1191490..1191909 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV259_RS05580	1187087..1187314	+	228	WP_124304273.1	hypothetical protein	-
HV259_RS05585	1188116..1188310	+	195	Protein_1082	hypothetical protein	-
HV259_RS05590	1188323..1189174	+	852	Protein_1083	RHS domain-containing protein	-
HV259_RS05595	1189171..1189653	+	483	WP_063139119.1	immunity 26/phosphotriesterase HocA family protein	-
HV259_RS05600	1189867..1190166	+	300	WP_124304274.1	hypothetical protein	-
HV259_RS05605	1190170..1190412	-	243	WP_124304275.1	hypothetical protein	-
HV259_RS05610	1190776..1191222	+	447	WP_045623318.1	hypothetical protein	-
HV259_RS05615	1191490..1191909	-	420	WP_045623284.1	helix-turn-helix domain-containing protein	Antitoxin
HV259_RS05620	1191906..1192217	-	312	WP_045623285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV259_RS05625	1192438..1192848	-	411	WP_023299534.1	ASCH domain-containing protein	-
HV259_RS05630	1193049..1193504	-	456	WP_023305643.1	hypothetical protein	-
HV259_RS05635	1194208..1194420	+	213	WP_022647355.1	RNA chaperone/antiterminator CspA	-
HV259_RS05640	1194647..1195543	-	897	WP_045623287.1	LysR family transcriptional regulator	-
HV259_RS05645	1195649..1196428	+	780	WP_088581799.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12453.32 Da
• Isoelectric Point: 9.8331

>T163842 WP_045623285.1 NZ_CP056663:c1192217-1191906 [Enterobacter hormaechei]
MPAVSRAPFDAATRHFPIQELALEDLYWVLKGEHYPSPDDMRKRFPSRERMKYREKWWVIDVGGGYLRVMLFTDFERGKL
FIKHTSTHADYDKLTDLYRRKKE

Nucleotide

• Length: 312 bp

>T163842 NZ_CP072764:677925-678332 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15522.56 Da
• Isoelectric Point: 4.7722

>AT163842 WP_045623284.1 NZ_CP056663:c1191909-1191490 [Enterobacter hormaechei]
MNFSAAIKAANDLTRELPFLGSSPSRQDYEDAMGLVEYLIEHDPDSPLVEMLTAKIDKYENESPEFAEFNARVASIPSGV
ALLRTLMDQYHLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGVQVSAFVGN

Nucleotide

• Length: 420 bp

>AT163842 NZ_CP072764:677713-677928 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA