Detailed information of TA system TA163838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5042826..5043586
• Replicon: chromosome
• Accession: NZ_CP056647
• Organism: Citrobacter sp. RHBSTW-00229

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: HV135_RS24500
• Protein ID: WP_019077936.1
• Coordinates: 5042826..5043137 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV135_RS24505
• Protein ID: WP_141876133.1
• Coordinates: 5043134..5043586 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV135_RS24470	5038493..5039395	+	903	WP_181220990.1	formate dehydrogenase O subunit beta	-
HV135_RS24475	5039392..5040027	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HV135_RS24480	5040024..5040953	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HV135_RS24485	5040990..5041364	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	-
HV135_RS24490	5041364..5041606	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	-
HV135_RS24495	5041812..5042741	+	930	WP_181220991.1	alpha/beta hydrolase	-
HV135_RS24500	5042826..5043137	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV135_RS24505	5043134..5043586	+	453	WP_141876133.1	helix-turn-helix domain-containing protein	Antitoxin
HV135_RS24510	5043604..5044545	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV135_RS24515	5044590..5045027	-	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV135_RS24520	5045024..5045896	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HV135_RS24525	5045890..5046489	-	600	WP_137351793.1	glucose-1-phosphatase	-
HV135_RS24530	5046757..5047785	-	1029	WP_047357782.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T163838 WP_019077936.1 NZ_CP056647:5042826-5043137 [Citrobacter sp. RHBSTW-00229]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T163838 NZ_CP072764:363476-363778 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16998.39 Da
• Isoelectric Point: 5.9259

>AT163838 WP_141876133.1 NZ_CP056647:5043134-5043586 [Citrobacter sp. RHBSTW-00229]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT163838 NZ_CP072764:363826-364047 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Toxin

• AlphaFold DB: A0A7L6U900