Detailed information of TA system TA163702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 78391..79118
• Replicon: plasmid pRHBSTW-00373_2
• Accession: NZ_CP056568
• Organism: Klebsiella oxytoca strain RHBSTW-00373

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HV180_RS29130
• Protein ID: WP_011251285.1
• Coordinates: 78807..79118 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV180_RS29125
• Protein ID: WP_011251286.1
• Coordinates: 78391..78810 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV180_RS29100	73918..74442	-	525	WP_029497499.1	hypothetical protein	-
HV180_RS29105	74635..75390	-	756	Protein_74	hypothetical protein	-
HV180_RS29110	75984..76448	+	465	WP_032435691.1	hypothetical protein	-
HV180_RS29115	76448..77134	+	687	Protein_76	hypothetical protein	-
HV180_RS29120	77278..78244	-	967	Protein_77	IS5 family transposase	-
HV180_RS29125	78391..78810	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HV180_RS29130	78807..79118	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV180_RS29135	79323..79781	-	459	Protein_80	DDE-type integrase/transposase/recombinase	-
HV180_RS29140	79874..80212	+	339	WP_047683432.1	IS66 family insertion sequence hypothetical protein	-
HV180_RS29145	80209..80556	+	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
HV180_RS29150	80605..81654	+	1050	Protein_83	IS66 family transposase	-
HV180_RS29155	81672..82564	-	893	Protein_84	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..202470	202470
	-	inside	IScluster/Tn	-	-	62427..86102	23675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T163702 WP_011251285.1 NZ_CP056568:c79118-78807 [Klebsiella oxytoca]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T163702 NZ_CP072761:2917307-2917684 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT163702 WP_011251286.1 NZ_CP056568:c78810-78391 [Klebsiella oxytoca]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT163702 NZ_CP072761:2917053-2917310 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1