Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2311702..2312264 Replicon chromosome
Accession NZ_CP056559
Organism Citrobacter freundii strain RHBSTW-00408

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag HV186_RS10940 Protein ID WP_096757033.1
Coordinates 2311986..2312264 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HV186_RS10935 Protein ID WP_096757032.1
Coordinates 2311702..2311986 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV186_RS10920 2307003..2310050 + 3048 WP_181491401.1 formate dehydrogenase-N subunit alpha -
HV186_RS10925 2310064..2310948 + 885 WP_115258486.1 formate dehydrogenase N subunit beta -
HV186_RS10930 2310941..2311597 + 657 WP_096757031.1 formate dehydrogenase-N subunit gamma -
HV186_RS10935 2311702..2311986 - 285 WP_096757032.1 HigA family addiction module antidote protein Antitoxin
HV186_RS10940 2311986..2312264 - 279 WP_096757033.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV186_RS10945 2312452..2314167 - 1716 WP_181491402.1 ATP-binding cassette domain-containing protein -
HV186_RS10950 2314478..2316175 - 1698 WP_115258488.1 malate dehydrogenase -
HV186_RS10955 2316355..2316495 - 141 WP_096757036.1 stationary-phase-induced ribosome-associated protein -
HV186_RS10960 2316724..2317155 + 432 WP_096757037.1 OsmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10601.05 Da        Isoelectric Point: 9.5320

>T163680 WP_096757033.1 NZ_CP056559:c2312264-2311986 [Citrobacter freundii]
MIMNFRHKGLRDLFLQGRTSGVTAKHVRRLRHRLAVIDAASHVTDINMPGYKLHALTGDRDGVWSISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T163680 NZ_CP056559:c2312264-2311986 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAAGGATTGCGCGACTTGTTTCTTCAGGGGCGAACATCAGGCGTGACGGCGAAGCATGT
CCGGCGATTGCGGCACCGACTTGCCGTTATTGATGCAGCGAGCCATGTAACAGATATCAATATGCCTGGTTATAAACTGC
ATGCGTTAACGGGCGATCGCGACGGCGTTTGGTCGATTTCTGTTTCAGGCAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGATGCATATATACTGGATTACGAGGACTATCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10327.05 Da        Isoelectric Point: 8.8094

>AT163680 WP_096757032.1 NZ_CP056559:c2311986-2311702 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAQ
KSVDISRLRKLVAQ

Download         Length: 285 bp

>AT163680 NZ_CP056559:c2311986-2311702 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGCCTGCGCGAATT
TGCCAGAGCAATGGAGATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAGGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGTTGAATCTGCAGAATGCCTGGAGTCTGGCTGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References