Detailed information of TA system TA163680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2311702..2312264
• Replicon: chromosome
• Accession: NZ_CP056559
• Organism: Citrobacter freundii strain RHBSTW-00408

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV186_RS10940
• Protein ID: WP_096757033.1
• Coordinates: 2311986..2312264 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV186_RS10935
• Protein ID: WP_096757032.1
• Coordinates: 2311702..2311986 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV186_RS10920	2307003..2310050	+	3048	WP_181491401.1	formate dehydrogenase-N subunit alpha	-
HV186_RS10925	2310064..2310948	+	885	WP_115258486.1	formate dehydrogenase N subunit beta	-
HV186_RS10930	2310941..2311597	+	657	WP_096757031.1	formate dehydrogenase-N subunit gamma	-
HV186_RS10935	2311702..2311986	-	285	WP_096757032.1	HigA family addiction module antidote protein	Antitoxin
HV186_RS10940	2311986..2312264	-	279	WP_096757033.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV186_RS10945	2312452..2314167	-	1716	WP_181491402.1	ATP-binding cassette domain-containing protein	-
HV186_RS10950	2314478..2316175	-	1698	WP_115258488.1	malate dehydrogenase	-
HV186_RS10955	2316355..2316495	-	141	WP_096757036.1	stationary-phase-induced ribosome-associated protein	-
HV186_RS10960	2316724..2317155	+	432	WP_096757037.1	OsmC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10601.05 Da
• Isoelectric Point: 9.5320

>T163680 WP_096757033.1 NZ_CP056559:c2312264-2311986 [Citrobacter freundii]
MIMNFRHKGLRDLFLQGRTSGVTAKHVRRLRHRLAVIDAASHVTDINMPGYKLHALTGDRDGVWSISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T163680 NZ_CP056559:c2312264-2311986 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAAGGATTGCGCGACTTGTTTCTTCAGGGGCGAACATCAGGCGTGACGGCGAAGCATGT
CCGGCGATTGCGGCACCGACTTGCCGTTATTGATGCAGCGAGCCATGTAACAGATATCAATATGCCTGGTTATAAACTGC
ATGCGTTAACGGGCGATCGCGACGGCGTTTGGTCGATTTCTGTTTCAGGCAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGATGCATATATACTGGATTACGAGGACTATCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10327.05 Da
• Isoelectric Point: 8.8094

>AT163680 WP_096757032.1 NZ_CP056559:c2311986-2311702 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAQ
KSVDISRLRKLVAQ

Nucleotide

• Length: 285 bp

>AT163680 NZ_CP056559:c2311986-2311702 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGCCTGCGCGAATT
TGCCAGAGCAATGGAGATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAGGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGTTGAATCTGCAGAATGCCTGGAGTCTGGCTGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA