Detailed information of TA system TA163668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 323136..323802
• Replicon: chromosome
• Accession: NZ_CP056559
• Organism: Citrobacter freundii strain RHBSTW-00408

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV186_RS01555
• Protein ID: WP_117342464.1
• Coordinates: 323485..323802 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: HV186_RS01550
• Protein ID: WP_003837894.1
• Coordinates: 323136..323432 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV186_RS01535	320312..320785	-	474	WP_005121627.1	transcription elongation factor GreB	-
HV186_RS01540	321012..321731	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HV186_RS01545	321728..323080	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
HV186_RS01550	323136..323432	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
HV186_RS01555	323485..323802	-	318	WP_117342464.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV186_RS01560	323926..325548	-	1623	WP_115257300.1	phosphoenolpyruvate carboxykinase (ATP)	-
HV186_RS01565	325927..327645	+	1719	WP_181490895.1	DUF4153 domain-containing protein	-
HV186_RS01570	327696..328574	-	879	WP_096759272.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12226.13 Da
• Isoelectric Point: 9.8310

>T163668 WP_117342464.1 NZ_CP056559:c323802-323485 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T163668 NZ_CP072761:703489-703890 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT163668 WP_003837894.1 NZ_CP056559:c323432-323136 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT163668 NZ_CP072761:703247-703492 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5