Detailed information of TA system TA163649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3142247..3142907
• Replicon: chromosome
• Accession: NZ_CP056546
• Organism: Citrobacter sp. RHBSTW-00424

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2I8S535
• Locus tag: HV191_RS15450
• Protein ID: WP_053390179.1
• Coordinates: 3142554..3142907 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV191_RS15445
• Protein ID: WP_103283718.1
• Coordinates: 3142247..3142549 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV191_RS15430	3138915..3140177	+	1263	WP_103283720.1	integrase arm-type DNA-binding domain-containing protein	-
HV191_RS15435	3140859..3141746	-	888	WP_103283719.1	integrase domain-containing protein	-
HV191_RS15440	3141878..3142180	+	303	WP_146053242.1	hypothetical protein	-
HV191_RS15445	3142247..3142549	-	303	WP_103283718.1	XRE family transcriptional regulator	Antitoxin
HV191_RS15450	3142554..3142907	-	354	WP_053390179.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV191_RS15455	3143406..3143627	-	222	WP_103283717.1	helix-turn-helix domain-containing protein	-
HV191_RS15460	3143624..3144091	-	468	WP_103283716.1	hypothetical protein	-
HV191_RS15465	3144472..3146076	-	1605	WP_103283715.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13442.36 Da
• Isoelectric Point: 9.0134

>T163649 WP_053390179.1 NZ_CP056546:c3142907-3142554 [Citrobacter sp. RHBSTW-00424]
VWTIKTTDMFDHWFSSLNDIDRASVLAALLVLREKGPGLSRPYADTLRGSRFSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMIPVADREFTNWLNTIKEKE

Nucleotide

• Length: 354 bp

>T163649 NZ_CP072742:c1623380-1623045 [Serratia fonticola]
ATGAAGGCCATCGACAAAATACCCCGCACTACACCTAACGCCAATCAGGCGTTGGCCGAATACCAGCAACTGCTTACCTG
GTTGCTGAACCACTGTTATGGATTAGAACTCAACGATACGCCGTATCACAACCCACAAGCTATCGCTCAACAGATTGAGT
ACGGAATGACAGTACGTGAAACCATCAACGAACTGGTGGAGAAATTCGACCTGGTACGCATTGACCGCCCCGGCTTCAGT
CTGCTGGCACAGGATCCGACGCTGAGTGGTGGGGATATGCTACGTGCCCGCAGTGCGCTAGGGCTGCGAAACCCCGTAAA
TCGCCGCCTCGCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11054.88 Da
• Isoelectric Point: 6.4738

>AT163649 WP_103283718.1 NZ_CP056546:c3142549-3142247 [Citrobacter sp. RHBSTW-00424]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAKALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDIELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT163649 NZ_CP072742:c1622968-1622609 [Serratia fonticola]
ATGAAAAACCTCAAACCTGATACCCCATCCACGTCCTCCGTCTGGGGTCTGCAATGTGCAATTACGCCACGTTTAGGAGC
TCGACTGGTCCAACAGGGCCGCCGCCTGCATTTTCTGGCTGACCGCTCCGGTTTGATCGGCACGTTCACACCCGAAGCCC
TCAAATCTCTCGATGTGGCTTTTCCGGTGCTGATTGAAAAGTTGGAGAACAATCTGCGCTCCGGTGAGCTCGATCCACGT
CGCCAGCATTGTGTCACTGTTCAGCACGCAGGATTTACCTGTGAAGCCGATACGTTGGGCAGTTTTGGCTATGTATATAT
CGCGGTCTATCAAGCGGCACCGTCACCAACTGCAAAGTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S535