Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 98307..99034 Replicon plasmid pRHBSTW-00444_2
Accession NZ_CP056516
Organism Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag HV198_RS23765 Protein ID WP_000558568.1
Coordinates 98307..98618 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag HV198_RS23770 Protein ID WP_000990392.1
Coordinates 98615..99034 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV198_RS23740 94858..95781 - 924 WP_000992320.1 aminoimidazole riboside kinase -
HV198_RS23745 96130..96381 + 252 Protein_106 transposase -
HV198_RS23750 96436..97140 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HV198_RS23755 97205..97666 + 462 Protein_108 lactate utilization protein C -
HV198_RS23760 97716..98096 - 381 Protein_109 gluconate permease -
HV198_RS23765 98307..98618 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
HV198_RS23770 98615..99034 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
HV198_RS23780 100367..100678 - 312 WP_001324897.1 hypothetical protein -
HV198_RS23785 100840..101190 + 351 WP_000928911.1 metalloregulator ArsR/SmtB family transcription factor -
HV198_RS23790 101495..101971 - 477 WP_000043177.1 DNA starvation/stationary phase protection protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3'')-Ib / aph(6)-Id - 1..216816 216816
- inside IScluster/Tn - - 96136..105839 9703


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T163612 WP_000558568.1 NZ_CP056516:98307-98618 [Citrobacter freundii]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T163612 NZ_CP072689:c619791-619450 [Escherichia marmotae]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAATCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCACGTAAACAAAAGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT163612 WP_000990392.1 NZ_CP056516:98615-99034 [Citrobacter freundii]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT163612 NZ_CP072689:619786-619862 [Escherichia marmotae]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References