Detailed information of TA system TA163593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 308456..309122
• Replicon: chromosome
• Accession: NZ_CP056515
• Organism: Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV198_RS01460
• Protein ID: WP_115257299.1
• Coordinates: 308805..309122 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: HV198_RS01455
• Protein ID: WP_003837894.1
• Coordinates: 308456..308752 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV198_RS01440	305632..306105	-	474	WP_005121627.1	transcription elongation factor GreB	-
HV198_RS01445	306332..307051	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HV198_RS01450	307048..308400	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
HV198_RS01455	308456..308752	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
HV198_RS01460	308805..309122	-	318	WP_115257299.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV198_RS01465	309246..310868	-	1623	WP_181506457.1	phosphoenolpyruvate carboxykinase (ATP)	-
HV198_RS01470	311247..312965	+	1719	WP_181506458.1	DUF4153 domain-containing protein	-
HV198_RS01475	313016..313894	-	879	WP_096759272.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12225.19 Da
• Isoelectric Point: 10.2614

>T163593 WP_115257299.1 NZ_CP056515:c309122-308805 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTKEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T163593 NZ_CP072687:c4568912-4568739 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT163593 WP_003837894.1 NZ_CP056515:c308752-308456 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT163593 NZ_CP072687:c4569236-4568940 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5