Detailed information of TA system TA163558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 314399..315065
• Replicon: chromosome
• Accession: NZ_CP056473
• Organism: Citrobacter freundii strain RHBSTW-00477

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV211_RS01475
• Protein ID: WP_117342464.1
• Coordinates: 314748..315065 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: HV211_RS01470
• Protein ID: WP_003837894.1
• Coordinates: 314399..314695 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV211_RS01455	311575..312048	-	474	WP_003023524.1	transcription elongation factor GreB	-
HV211_RS01460	312275..312994	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HV211_RS01465	312991..314343	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
HV211_RS01470	314399..314695	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
HV211_RS01475	314748..315065	-	318	WP_117342464.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV211_RS01480	315189..316811	-	1623	WP_181631795.1	phosphoenolpyruvate carboxykinase (ATP)	-
HV211_RS01485	317190..318908	+	1719	WP_181631796.1	DUF4153 domain-containing protein	-
HV211_RS01490	318959..319837	-	879	WP_096759272.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12226.13 Da
• Isoelectric Point: 9.8310

>T163558 WP_117342464.1 NZ_CP056473:c315065-314748 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T163558 NZ_CP072683:c2859062-2858772 [Pseudoalteromonas sp. SCSIO 43201]
ATGACCTATAAACTCGACTTTAAAAAGAGCGCACTCAAAGAGTGGAAAAAGCTTGGCTCTACTCTACAACAACAATTTAA
GAAAAAGCTAATCGAGCGCTTAGATAACCCACATGTTCCGGCTTCAAAGCTTTCTGGAGCTGACAACATGTATAAAATTA
AGTTGCGTCAATCGGGCTACCGTCTCGTCTACAAAGTTGAAGACGATGTCATCATTGTAACCGTCTTAGCAGTCGGAAAA
CGCGAACGTAGCGATGTTTACCGTAAAGCTATGAAAAGGCTAGATGATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT163558 WP_003837894.1 NZ_CP056473:c314695-314399 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT163558 NZ_CP072683:c2859300-2859052 [Pseudoalteromonas sp. SCSIO 43201]
ATGACCACTAGAATTTTAGCCGATGTTGCTGCAAGCATTACTGAGTTGAAAGCTAACCCTATGAAAGTTGCAACTAGTGC
TTATGGCGAACCTGTTGCTGTACTAAACCGAAATGAGCCGGCATTTTATTGCGTACCTGCCGAAGCGTACGAAATGATGA
TGGACAGACTCGAAGATCTTGAACTACTAGCTATCGCTAAAGAGCGTGAATCTGAAGAAAGCATTTCGGTAAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5