Detailed information of TA system TA163555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4591548..4592218
• Replicon: chromosome
• Accession: NZ_CP056466
• Organism: Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: HV214_RS22325
• Protein ID: WP_046671374.1
• Coordinates: 4591916..4592218 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV214_RS22320
• Protein ID: WP_046671375.1
• Coordinates: 4591548..4591889 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV214_RS22290	4587089..4587847	+	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-
HV214_RS22295	4587937..4588617	+	681	WP_046671387.1	phosphonate C-P lyase system protein PhnL	-
HV214_RS22300	4588614..4589750	+	1137	WP_003031811.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HV214_RS22305	4589753..4590307	+	555	WP_003844764.1	ribose 1,5-bisphosphokinase	-
HV214_RS22310	4590294..4590728	+	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
HV214_RS22315	4590737..4591495	+	759	WP_046671376.1	phosphonate metabolism protein PhnP	-
HV214_RS22320	4591548..4591889	-	342	WP_046671375.1	HigA family addiction module antidote protein	Antitoxin
HV214_RS22325	4591916..4592218	-	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV214_RS22330	4592374..4592703	-	330	WP_003031807.1	hypothetical protein	-
HV214_RS22335	4592773..4595037	-	2265	WP_044702173.1	hybrid sensor histidine kinase/response regulator	-
HV214_RS22340	4595152..4596675	+	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T163555 WP_046671374.1 NZ_CP056466:c4592218-4591916 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T163555 NZ_CP072680:1423906-1424160 [Vibrio sp. SCSIO 43186]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTTTGGACGGTGTAGTTAATGACGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAAGATGCTGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACCGTTCACT
TACTACGCTCTCCTCAAAACGCTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAACACGAGAG
TTAATTGATGAGTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT163555 WP_046671375.1 NZ_CP056466:c4591889-4591548 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT163555 NZ_CP072680:1424153-1424422 [Vibrio sp. SCSIO 43186]
ATGAGTAGTAGTCAACGTTTACTATCGTGGACTGATGACGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAACAAACTCATCAATGATGTAAAGCGCTCCCCATTTGAGGGTATCGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTGTTGATGACACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATCTCGTGTCGTTACCACTACTAA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6