Detailed information of TA system TA163535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 34659..35307
• Replicon: chromosome
• Accession: NZ_CP056466
• Organism: Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV214_RS00170
• Protein ID: WP_046671055.1
• Coordinates: 34659..35021 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0D7LEH2
• Locus tag: HV214_RS00175
• Protein ID: WP_003840660.1
• Coordinates: 35008..35307 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV214_RS00155	31118..32446	+	1329	WP_003023927.1	MFS transporter	-
HV214_RS00160	32589..33980	+	1392	WP_003023929.1	hexose-6-phosphate:phosphate antiporter	-
HV214_RS00165	34106..34558	+	453	WP_003023933.1	DUF1198 family protein	-
HV214_RS00170	34659..35021	+	363	WP_046671055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV214_RS00175	35008..35307	+	300	WP_003840660.1	helix-turn-helix transcriptional regulator	Antitoxin
HV214_RS00180	35426..36616	+	1191	WP_181212909.1	purine ribonucleoside efflux pump NepI	-
HV214_RS00185	36666..38048	-	1383	WP_046671054.1	glycoside hydrolase family 1 protein	-
HV214_RS00190	38143..38436	-	294	WP_003840665.1	hypothetical protein	-
HV214_RS00195	38567..38983	+	417	WP_003023951.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	34659..65490	30831

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13814.69 Da
• Isoelectric Point: 4.9208

>T163535 WP_046671055.1 NZ_CP056466:34659-35021 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRYGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Nucleotide

• Length: 363 bp

>T163535 NZ_CP072653:754129-754539 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATTCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11016.85 Da
• Isoelectric Point: 8.5047

>AT163535 WP_003840660.1 NZ_CP056466:35008-35307 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Nucleotide

• Length: 300 bp

>AT163535 NZ_CP072653:753882-754148 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0D7LEH2