Detailed information of TA system TA163513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2530293..2530855
• Replicon: chromosome
• Accession: NZ_CP056451
• Organism: Citrobacter freundii strain RHBSTW-00502

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV221_RS12310
• Protein ID: WP_032936429.1
• Coordinates: 2530577..2530855 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B5QC60
• Locus tag: HV221_RS12305
• Protein ID: WP_003836231.1
• Coordinates: 2530293..2530577 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV221_RS12290	2527984..2528868	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
HV221_RS12295	2528861..2529517	+	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
HV221_RS12300	2529647..2530249	+	603	WP_032935729.1	inorganic diphosphatase	-
HV221_RS12305	2530293..2530577	-	285	WP_003836231.1	HigA family addiction module antidote protein	Antitoxin
HV221_RS12310	2530577..2530855	-	279	WP_032936429.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV221_RS12315	2531076..2532791	-	1716	WP_032935731.1	ATP-binding cassette domain-containing protein	-
HV221_RS12320	2533101..2534798	-	1698	WP_003020042.1	malate dehydrogenase	-
HV221_RS12325	2534978..2535118	-	141	WP_003020039.1	stationary-phase-induced ribosome-associated protein	-
HV221_RS12330	2535346..2535777	+	432	WP_003020036.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10667.30 Da
• Isoelectric Point: 9.8965

>T163513 WP_032936429.1 NZ_CP056451:c2530855-2530577 [Citrobacter freundii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGDRDGIWAITVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T163513 NZ_CP072628:479937-480185 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10358.10 Da
• Isoelectric Point: 8.8094

>AT163513 WP_003836231.1 NZ_CP056451:c2530577-2530293 [Citrobacter freundii]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQTTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT163513 NZ_CP072628:480179-480502 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTAGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B5QC60