Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 357529..358175 Replicon chromosome
Accession NZ_CP056432
Organism Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag HV225_RS01655 Protein ID WP_016529833.1
Coordinates 357529..357876 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag HV225_RS01660 Protein ID WP_002920557.1
Coordinates 357876..358175 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV225_RS01645 353455..354888 + 1434 WP_002920564.1 glycogen synthase GlgA -
HV225_RS01650 354906..357353 + 2448 WP_002920561.1 glycogen phosphorylase -
HV225_RS01655 357529..357876 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV225_RS01660 357876..358175 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
HV225_RS01665 358238..359746 - 1509 WP_023301456.1 glycerol-3-phosphate dehydrogenase -
HV225_RS01670 359951..360280 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
HV225_RS01675 360331..360774 + 444 Protein_330 rhomboid family intramembrane serine protease GlpG -
HV225_RS01680 360833..361876 + 1044 WP_116280962.1 IS481-like element ISKpn28 family transposase -
HV225_RS01685 361867..362262 + 396 Protein_332 rhomboid family intramembrane serine protease -
HV225_RS01690 362312..363070 + 759 WP_032456686.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 361382..361876 494


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T163479 WP_016529833.1 NZ_CP056432:357529-357876 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T163479 NZ_CP072605:645992-646330 [Burkholderia latens]
ATGGTTGACATACTGAAATCCATGCCCTACAGTTCGCCTTCGTACAGTATCCGGACCACAGACGTCTTCGACGCGTGGTT
TGCCGCCCTTACGGATCGCGTCGCGCGGCGCCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAACCCGGGCGACT
GGAAATCCGCCGGCTCACCGATCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTACAACGCGAGGCGCGAA
ACGATATGGGTGATCCTGCTCTGCGGCGGTGACAAGTCGACACAACAGGCGGACATCCGGGGCGCGCACGCGATGCTCGA
ACGGCTCGACCTGGAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT163479 WP_002920557.1 NZ_CP056432:357876-358175 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT163479 NZ_CP072605:646334-646633 [Burkholderia latens]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGACGACGACATGGCCGAGTATTTCGAGGC
CTGTCTGCAGGAAGCCGGCGACGACCCGGCCTTCATCGCGCATGCGCTTGGCGTGATCGCGCGCGCGCGCGGGATGTCGC
ATGTCGCGCGCGACGCCGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAGCTGCACGGCGCGAAGGCGCACGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References