Detailed information of TA system TA163455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5078545..5079305
• Replicon: chromosome
• Accession: NZ_CP056421
• Organism: Citrobacter sp. RHBSTW-00524

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: HV229_RS24310
• Protein ID: WP_019077936.1
• Coordinates: 5078545..5078856 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV229_RS24315
• Protein ID: WP_104651900.1
• Coordinates: 5078853..5079305 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV229_RS24280	5074207..5075109	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HV229_RS24285	5075106..5075741	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HV229_RS24290	5075738..5076667	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HV229_RS24295	5076711..5076998	-	288	WP_131347314.1	helix-turn-helix domain-containing protein	-
HV229_RS24300	5076999..5077310	-	312	WP_131347312.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV229_RS24305	5077531..5078460	+	930	WP_181546576.1	alpha/beta hydrolase	-
HV229_RS24310	5078545..5078856	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV229_RS24315	5078853..5079305	+	453	WP_104651900.1	helix-turn-helix domain-containing protein	Antitoxin
HV229_RS24320	5079323..5080264	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV229_RS24325	5080309..5080746	-	438	WP_016157874.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV229_RS24330	5080743..5081615	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HV229_RS24335	5081609..5082208	-	600	WP_016157873.1	glucose-1-phosphatase	-
HV229_RS24340	5082299..5083189	-	891	WP_181546578.1	aldose 1-epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5076711..5090892	14181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T163455 WP_019077936.1 NZ_CP056421:5078545-5078856 [Citrobacter sp. RHBSTW-00524]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T163455 NZ_CP072580:19122-19472 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 17028.41 Da
• Isoelectric Point: 5.9259

>AT163455 WP_104651900.1 NZ_CP056421:5078853-5079305 [Citrobacter sp. RHBSTW-00524]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVTLLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT163455 NZ_CP072580:18865-19128 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A7L6U900