Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4865556..4866316 Replicon chromosome
Accession NZ_CP056409
Organism Citrobacter sp. RHBSTW-00535

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3E2Q1
Locus tag HV232_RS23170 Protein ID WP_047357780.1
Coordinates 4865556..4865867 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7W3E2R1
Locus tag HV232_RS23175 Protein ID WP_047357781.1
Coordinates 4865864..4866316 (+) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV232_RS23140 4861223..4862125 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV232_RS23145 4862122..4862757 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV232_RS23150 4862754..4863683 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV232_RS23155 4863720..4864094 - 375 WP_016155182.1 type II toxin-antitoxin system VapC family toxin -
HV232_RS23160 4864094..4864336 - 243 WP_016155181.1 ribbon-helix-helix domain-containing protein -
HV232_RS23165 4864542..4865471 + 930 WP_094169176.1 alpha/beta hydrolase -
HV232_RS23170 4865556..4865867 + 312 WP_047357780.1 type II toxin-antitoxin system HigB family toxin Toxin
HV232_RS23175 4865864..4866316 + 453 WP_047357781.1 helix-turn-helix domain-containing protein Antitoxin
HV232_RS23180 4866334..4867275 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV232_RS23185 4867320..4867757 - 438 WP_016157874.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV232_RS23190 4867754..4868626 - 873 WP_019077938.1 virulence factor BrkB family protein -
HV232_RS23195 4868620..4869219 - 600 WP_016157873.1 glucose-1-phosphatase -
HV232_RS23200 4869487..4870515 - 1029 WP_047357782.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(95-148)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12545.70 Da        Isoelectric Point: 10.1490

>T163437 WP_047357780.1 NZ_CP056409:4865556-4865867 [Citrobacter sp. RHBSTW-00535]
MHVISRKPFNEARLRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Download         Length: 312 bp

>T163437 NZ_CP072566:2269212-2269499 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTAAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16986.38 Da        Isoelectric Point: 6.1421

>AT163437 WP_047357781.1 NZ_CP056409:4865864-4866316 [Citrobacter sp. RHBSTW-00535]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQHGLKAADLANELGSKSNVSNILNGRRALTISHIKMLAERFNLPADAFIE

Download         Length: 453 bp

>AT163437 NZ_CP072566:2268973-2269212 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E2Q1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3E2R1

References